Mouse anti-Human CD15/SSEA-1 Monoclonal Antibody for Flow Cytometry - P22083

Core Product Specifications and Parameters

Biological Background: Alpha-(1,3)-fucosyltransferase 4 (FUT4) Function and Localization

• Alpha-(1,3)-fucosyltransferase 4 (FUT4) is a Golgi-resident glycosyltransferase that synthesizes the Lewis X (LeX) glycan determinant (CD15) by transferring fucose in alpha(1,3)-linkage to N-acetylglucosamine (GlcNAc) on type 2 lactosamine chains. Related references: PMID:1702034, PMID:1716630, PMID:29593094
• CD15/LeX is a key cell-surface glycoepitope involved in cell adhesion, migration, and immune cell recognition, particularly through interactions with selectins (SELE, SELL, SELP) during leukocyte trafficking and homing.
• FUT4 works together with FUT7 to decorate selectin ligands with fucosylated glycans, a critical step in lymphocyte recirculation and inflammatory responses.
• The enzyme can also modify sialylated acceptors to generate sialyl Lewis X and 6‑sulfo sialyl Lewis X determinants, which serve as high-affinity ligands for the same selectin family. Related references: PMID:1716630, PMID:29593094
• FUT4 localizes exclusively to the medial/trans‑Golgi membrane via an N‑terminal signal‑anchor, compatible with its role in terminal glycan processing.
• Tissue expression includes mesenchymal stem cells, myeloid and lymphoid lineages, and immature promyelocytes, linking CD15 to both hematopoietic differentiation and stem cell biology.
• Additionally, FUT4 may fucosylate internal LacNAc units to form the VIM‑2 epitope, another selectin‑binding structure, further diversifying cell‑surface glycan repertoires. Related references: PMID:11278338, PMID:1716630

Experimental Guidance and Technical Tips

• This -conjugated antibody is ready-to-use for direct flow cytometry; titrate the antibody from 0.5–5 µg/mL to determine optimal staining concentration for your cell type.
• Use live, unfixed human cells (e.g., peripheral blood leukocytes or cultured cell lines) to preserve the native carbohydrate epitope, as fixation may alter CD15 recognition.
• Include appropriate isotype control and use Fc receptor blocking to assess non-specific binding.
• If intracellular Golgi staining is required, permeabilization protocols must be optimized to retain membrane structures.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio streamlines procurement of validated antibodies, bulk ancillary reagents, and rare-target sourcing for glycobiology and leukocyte marker research. Whether you are developing flow cytometry panels for stem cell or immunophenotyping applications, our supply portfolio encompasses optimized monoclonal and polyclonal antibodies, supporting your workflow from concept to clinic.