Mouse anti-Human CD11c Monoclonal Antibody for FC - P20702

Core Product Specifications and Parameters

Biological Background: CD11c Function and Localization

• CD11c, also known as integrin alpha-X, is a leukocyte adhesion glycoprotein that pairs with integrin beta-2 to form the p150,95 receptor.
• The CD11c/beta-2 integrin binds fibrinogen by recognizing the G-P-R sequence, mediating cell-cell adhesion during inflammatory responses and particularly regulating monocyte adhesion and chemotaxis.
• CD11c serves as a receptor for the erythrocyte-specific adhesion molecule ICAM4, facilitating erythrophagocytosis. Related references: PMID:16985175
• Subcellularly, CD11c localizes to the cell membrane, consistent with its role in cell surface adhesion.
• Tissue expression is predominantly in monocytes and granulocytes, underscoring its importance in innate immune cell functions.
• CD11c belongs to the integrin alpha chain family, characterized by extracellular repeats, and its activity depends on divalent cations such as calcium and magnesium. Post-translational modifications include disulfide bonds and glycosylation.

Experimental Guidance and Technical Tips

• The immunogen used was a recombinant full-length human CD11c protein; therefore, the antibody likely recognizes native conformations and may have epitopes within the extracellular domain.
• This antibody is validated for flow cytometry (FC). For optimal performance, consider titrating the antibody in your experimental system and using appropriate isotype controls and viability dyes.
• Since CD11c is highly expressed on monocytes and granulocytes, validate staining in human peripheral blood mononuclear cells (PBMCs) or whole blood samples. Cell surface expression may be affected by activation or differentiation states.
• If combining with other fluorophores, ensure compensation controls are properly set given the brightness of .

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our offering includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing, enabling streamlined development of immunology and inflammation assays. For this CD11c antibody, we can supply matched reagents or custom conjugation services to meet your manufacturing scale-up needs.