MonoMethyl-UHRF1-K385 Rabbit pAb (Q96T88) - Epigenetics Antibody for WB, DB, ELISA

Core Product Specifications and Parameters

Biological Background: UHRF1 Function and Localization

• UHRF1 (E3 ubiquitin-protein ligase UHRF1) is a key epigenetic regulator that bridges DNA methylation and chromatin modification. It plays a critical role in the inheritance of DNA methylation patterns by recruiting DNMT1 to hemimethylated DNA during replication, thereby ensuring faithful propagation of methylation marks. Related references: PMID:10646863, PMID:15009091, PMID:19056828, PMID:23022729, PMID:24013172, PMID:27595565, PMID:30104358, PMID:30392929, PMID:30392931, PMID:39607687.
• Acting as both a histone reader and writer, UHRF1 specifically recognizes H3K9me3 and H3R2me0, which activates its E3 ubiquitin ligase activity. It then monoubiquitinates histone H3 at Lys-18 (H3K18ub), creating a docking site for DNMT1. It also ubiquitinates H3K14 and H3K23 to a lesser extent. Related references: PMID:15361834, PMID:17673620, PMID:17967883, PMID:18772889, PMID:21745816, PMID:21777816, PMID:22100450, PMID:22837395, PMID:23022729, PMID:27595565, PMID:30104358, PMID:24013172.
• In addition to its role in DNA methylation, UHRF1 participates in DNA repair by cooperating with UHRF2 to ensure FANCD2 recruitment to interstrand cross-links. It also ubiquitinates non-histone proteins such as KIF11 and PML, influencing spindle architecture and other processes. Related references: PMID:30335751, PMID:22945642, PMID:37728657.
• UHRF1 localizes predominantly to the nucleus and associates with chromosomes during S phase, particularly at replicating pericentric heterochromatin. In non-S-phase cells, it distributes homogeneously through the nucleus. Related references: PMID:10646863, PMID:17673620, PMID:17967883, PMID:21777816.
• Tissue expression is observed in thymus, bone marrow, testis, lung, and heart, with overexpression reported in breast cancer. Related references: PMID:10646863, PMID:12838312, PMID:15361834.

Experimental Guidance and Technical Tips

• For Western blot detection, consider enriching nuclear extracts or chromatin fractions to improve signal for UHRF1 and its methylation variants.
• As the antibody specifically recognizes monomethylated K385, use unmodified and differentially methylated peptide controls in dot blot (DB) or ELISA assays to confirm specificity.
• UHRF1 expression and modification are cell-cycle dependent; synchronizing cells to S phase may enhance detection of the target.
• The antibody is reported to cross-react with mouse and rat UHRF1; however, validate in the relevant species and sample type to ensure expected performance.
• Optimize blocking and washing conditions, as methylation-specific antibodies can require careful adjustment to minimize non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive one-stop sourcing platform for IVD raw materials, covering every stage from concept to clinic. This MonoMethyl-UHRF1-K385 rabbit polyclonal antibody supports epigenetics research, particularly DNA methylation and chromatin studies. Beyond antibodies, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your diagnostic development.