MonoMethyl-NONO/p54nrb (R357/R365) Rabbit Polyclonal Antibody (Q15233) for WB, IF/ICC, ELISA

Core Product Specifications and Parameters

Biological Background: NONO/p54nrb Function and Localization

NONO (Non-POU domain-containing octamer-binding protein), also known as p54nrb, is a multifunctional DNA- and RNA-binding protein involved in diverse nuclear processes. It binds both double- and single-stranded DNA and RNA, participates in pre-mRNA splicing likely as a heterodimer with SFPQ, and interacts with U5 snRNA. NONO is essential for paraspeckle formation together with PSPC1, and it contributes to DNA repair via non-homologous end joining (NHEJ) by stabilizing DNA ends with the Ku70/Ku80 dimer.

In transcriptional regulation, NONO forms a complex with SFPQ and NR5A1 to modulate CYP17 promoter activity. It also regulates the circadian clock by repressing CLOCK-BMAL1 transactivation, influences GABAergic synapse organization, and promotes neuronal differentiation by recruiting TET1 to genomic loci.

NONO plays a critical role in innate immunity by assembling into the HDP-RNP complex, which serves as a platform for IRF3 phosphorylation, thereby activating the cGAS-STING pathway in response to DNA virus infection. During HIV-2 infection, NONO interacts with the viral capsid protein p24 to facilitate viral DNA sensing by cGAS.

Subcellularly, NONO is predominantly nuclear, concentrating in nucleoli, nuclear speckles, and punctate paraspeckles adjacent to splicing speckles.

NONO is widely expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, and various breast tumor cell lines. The protein undergoes extensive post-translational modifications, including acetylation, phosphorylation, and methylation (e.g., monomethylation at R456). This antibody specifically recognizes the mono-methylated form at arginines 357 and 365.

Related references: PMID:11525732, PMID:12403470, PMID:26571461, PMID:22416126, PMID:15590677, PMID:11897684, PMID:28712728, PMID:30270045, PMID:9341872, PMID:19608861, PMID:24129315

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against a synthetic peptide carrying monomethylation at R357 and R365 of human NONO/p54nrb. For Western blot, a band around 54 kDa is expected. Validate specificity by comparing signal intensity with non-methylated peptide competition or by using methylation-specific controls.
• For IF/ICC, nuclear staining with enrichment in foci (paraspeckles) may be observed. Consider co-staining with paraspeckle markers (e.g., PSPC1) to confirm localization.
• The antibody is stated to react with human and rat samples. Cross-reactivity with other species is not tested; verify in your model system.
• Optimal working dilutions must be determined empirically for each application. Start with 1:500–1:1000 for WB and 1:50–1:200 for IF/ICC, adjusting as needed.
• The antibody is provided as liquid with ice bag shipping; store at −20°C upon receipt. Avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized rare-target development. This methyl-specific NONO/p54nrb antibody addresses the niche of arginine methylation research in RNA processing, DNA repair, and innate immunity, complementing our growing portfolio of epigenetic tools. From concept to clinic, CamelBio accelerates your diagnostic and research workflows with reliable supply and technical support.