MonoMethyl-Histone H4-K20 Rabbit Monoclonal Antibody (P62805) for WB, DB, IHC-P, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H4 Function and Localization

Histone H4 is a core component of the nucleosome, the fundamental unit of chromatin. Together with histones H2A, H2B, and H3, it forms an octamer around which DNA is wrapped, regulating access to genetic information. This compaction is essential for DNA organization within the nucleus and plays a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.

Related references: UniProt P62805

Histone H4 is predominantly localized to the nucleus and chromosomes. During spermatogenesis, a specific acetylated form of H4 is transiently localized to the nucleus in step 11 spermatids, highlighting the dynamic regulation of histone modification across cell types.

Related references: UniProt P62805

Histone H4 undergoes extensive post-translational modifications (PTMs), including acetylation, methylation, phosphorylation, citrullination, and ubiquitination, among others. These modifications, often referred to as the "histone code," are critical for modulating chromatin structure and function. Mono-methylation of lysine 20 (H4K20me1) is a conserved epigenetic mark implicated in transcriptional regulation, cell cycle progression, and DNA damage response.

Related references: UniProt P62805

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a synthetic monomethylated peptide spanning lysine 20 (K20) of human Histone H4 (NP_003539.1). When developing similar modification-specific antibodies, consider using a peptide with the modification of interest flanked by native sequences to ensure specificity.
• According to the supplier, this antibody has been validated for Western blotting (WB), dot blot (DB), immunohistochemistry on paraffin sections (IHC-P), and ELISA. For WB, expect a specific band approximately at 11 kDa corresponding to Histone H4. However, actual performance may vary depending on sample preparation and assay conditions; validation in the relevant sample system is recommended.
• Cross-reactivity is reported for human, mouse, rat, and other species (wide range predicted). While this suggests broad utility, users should verify reactivity in their specific model organism, especially for non-mammalian species.
• Since this antibody targets a specific post-translational modification, it is important to include appropriate controls (e.g., unmodified peptide, untreated samples, or si/shRNA knockdown) to confirm specificity in each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your strategic partner for IVD raw-material sourcing, offering a curated portfolio that includes this MonoMethyl-Histone H4-K20 Rabbit mAb for epigenetic and chromatin research. We support diagnostic developers with validated antibody pairs, bulk ancillary reagents, and flexible access to rare-target materials, ensuring you can move smoothly from assay concept to clinical application.