MonoMethyl-Histone H4-K20 (P62805) Rabbit Polyclonal Antibody for Epigenetic Research, WB/ELISA

Core Product Specifications and Parameters

Biological Background: Histone H4 Function and Localization

Histone H4 is a core component of the nucleosome, wrapping and compacting DNA into chromatin. This limits DNA accessibility and plays a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications, collectively termed the histone code, and nucleosome remodeling. Related references: UniProt P62805

Histone H4 localizes to the nucleus and is a structural component of chromosomes. Acetylated H4 is detected in the nucleus of step 11 spermatids. Related references: UniProt P62805

Lysine 20 of histone H4 (corresponding to position 21 in the full-length protein) is subject to mono-, di-, and tri-methylation. Monomethylation at H4K20 has been implicated in chromatin compaction, heterochromatin formation, and DNA damage response. These modifications add functional diversity to the histone code. Related references: PMID:12086618, PMID:15964846, PMID:17967882

Histone H4 is highly conserved across species, with a wide range of other post-translational modifications including acetylation, citrullination, phosphorylation, and ubiquitination at multiple residues. This conservation makes H4 a valuable target for studying epigenetic regulation in diverse model organisms. Related references: UniProt P62805

Experimental Guidance and Technical Tips

• The immunogen is a synthetic monomethylated peptide around K20; consider verifying antibody specificity by peptide competition ELISA using unmodified, mono-, di-, and tri-methylated K20 peptides.
• For Western blot, acid extraction of histones or nuclear fractionation is recommended to enrich for histone H4. The predicted molecular weight is approximately 11 kDa; use a 15% SDS-PAGE gel or gradient gel for optimal separation of low-molecular-weight histones.
• Given the broad predicted cross-reactivity (human, mouse, rat, and others), validate the antibody in the relevant sample system, as signal intensity may vary depending on endogenous methylation levels.
• An ELISA assay can be used to quantify relative monomethyl-H4K20 levels using standardized histone preparations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing bridge for IVD raw materials, offering this MonoMethyl-Histone H4-K20 Rabbit pAb alongside a comprehensive portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Researchers and diagnostic developers focusing on epigenetic and chromatin biology can efficiently procure rare-target reagents, streamlining their transition from concept to clinical application. Contact CamelBio for tailored sourcing support in histone modification and epigenetics research.