MonoMethyl-Histone H3-R8 (Q16695/P68431) Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H3.1t/H3.1 Function and Localization

Histone H3 is a core component of the nucleosome, wrapping DNA into chromatin and limiting accessibility for transcription, replication, and repair. Post-translational modifications (PTMs) of histone tails constitute a "histone code" that regulates these processes.
Related references: UniProt: Q16695, P68431.

The human genome encodes multiple H3 variants: H3.1 is ubiquitous and encoded by several genes (H3C1–H3C12), while H3.1t (H3-4/HIST3H3) is testis-specific.
Related references: UniProt: Q16695.

Arginine 8 (R8) in the N‑terminal tail can undergo monomethylation (as targeted by this antibody), symmetric dimethylation (by PRMT5), or citrullination. These marks influence chromatin states and gene expression.
Related references: PMID:15345777, PMID:16567635.

Histone H3 localizes to the nucleus and chromosomes, with a predicted molecular weight of approximately 15 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic monomethylated peptide around arginine 8; the antibody should primarily detect monomethyl‑H3R8. Verify specificity with control lysates/peptides to exclude cross‑reactivity with symmetric dimethylation or citrullination at the same site.
• For Western blotting, histone enrichment (acid extraction) often improves signal; the expected band is ~15 kDa.
• In IHC‑P, heat‑induced antigen retrieval and testis tissue (rich in H3.1t) are recommended as positive controls.
• For IF/ICC, optimize fixation and permeabilization since the target is nuclear.
• ELISA assays may benefit from including a monomethyl‑peptide standard for calibration.
• Predicted to react across human, mouse, rat, and other species due to sequence conservation; however, validation in the relevant sample system is advised.
• As a polyclonal antibody, lot‑to‑lot consistency may vary; always re‑titrate and validate new lots.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined one‑stop sourcing platform for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. The MonoMethyl‑Histone H3‑R8 antibody is part of our expanding portfolio of epigenetics tools. Beyond single reagents, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can assist with hard‑to‑find raw‑material sourcing to accelerate your assay development.