MonoMethyl-Histone H3-R26 Rabbit pAb – Q16695/P68431 for WB, IF/ICC, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

• Histone H3 is a core component of the nucleosome, playing a central role in DNA packaging and chromatin organization. It is critical for processes such as transcription, replication, and DNA repair. Related references: UniProt Q16695, UniProt P68431
• Histone H3 localizes predominantly to the nucleus and is associated with chromosomes. Related references: UniProt Q16695, UniProt P68431
• Post-translational modifications on histone H3, collectively referred to as the histone code, regulate gene activity and chromatin states. Important modifications include methylation at various arginine and lysine residues. For instance, arginine residues can be monomethylated, symmetrically or asymmetrically dimethylated by protein arginine methyltransferases, influencing transcriptional outcomes (e.g., R3 methylation by PRMT6, PMID: 17898714, PMID: 18077460; R17 methylation by CARM1, PMID: 15345777). Monomethylation at R26 is a specific modification of interest. Related references: PMID:17898714, PMID:18077460, PMID:15345777
• Histone H3 variants exhibit tissue-specific expression: H3.1t (Q16695) is primarily expressed in testicular cells, whereas canonical H3.1 (P68431) is ubiquitous. Related references: UniProt Q16695 (tissue specificity statement).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic monomethylated peptide around arginine 26 of human histone H3. When using this antibody, consider that it may preferentially recognize the monomethylated form; cross-reactivity with unmethylated or other methylated states (e.g., dimethyl) should be validated in your specific experimental system.
• For Western blot (WB), nuclear-enriched lysates are recommended to enhance detection of histone H3. Optimize loading and transfer conditions appropriate for low-molecular-weight (~15 kDa) proteins.
• In immunofluorescence/immunocytochemistry (IF/ICC), permeabilization methods and fixation can affect epitope accessibility; validate the staining pattern in appropriate positive and negative controls.
• For ELISA, the antibody can be used to detect monomethylated H3 peptides; consider using competitive assays to confirm specificity.
• As reactivity is predicted for a wide range of species beyond human, mouse, and rat, validation in other species is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For projects targeting epigenetic modifications such as histone methylation, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents. Our supply chain supports bulk procurement of high-quality raw materials, including this monomethyl-histone H3-R26 antibody, ensuring seamless integration into your assay development and manufacturing workflows.