MonoMethyl-Histone H3-R2 Rabbit Polyclonal Antibody (Q16695/P68431) | WB, IF/ICC, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H3 (H3.1/H3.1t) Function and Localization

• Histone H3 is a core component of the nucleosome, wrapping and compacting DNA into chromatin. This limits DNA accessibility and plays a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is controlled through the "histone code" — a complex set of post-translational modifications (PTMs) and nucleosome remodeling.
• The protein localizes to the nucleus and chromosomes.
• Two major isoforms are relevant: H3.1 (encoded by multiple genes, e.g., H3C1, H3C2, etc., UniProt P68431) is expressed in most somatic cells, whereas H3.1t (H3-4, HIST3H3, UniProt Q16695) is testis-specific.
• The N-terminal tail of histone H3 is subject to numerous PTMs, including acetylation, methylation, phosphorylation, citrullination, and others. Specifically, arginine 2 (R2) can be methylated. Asymmetric dimethylation at this site is catalyzed by PRMT6 and is associated with transcriptional regulation. Monomethylation at R2 is an intermediate step and may have distinct functional roles. This antibody specifically recognizes the monomethylated form of R2. Related references: PMID:17898714, PMID:18077460, PMID:18079182

Experimental Guidance and Technical Tips

• The immunogen is a synthetic monomethylated peptide around R2 of human histone H3. When performing western blot (WB), consider using nuclear extracts or histone-enriched preparations to increase signal. The predicted molecular weight is ~15 kDa, but bands may appear slightly higher due to PTMs or protein interactions.
• For immunofluorescence/immunocytochemistry (IF/ICC), fixation and permeabilization conditions may need optimization. Since histone H3 is nuclear, permeabilization with Triton X-100 or methanol is recommended. Validate the staining pattern against known nuclear markers.
• In ELISA, consider using a methyl-peptide as the coating antigen to confirm specificity for monomethyl R2 versus unmodified or dimethylated forms.
• Species cross-reactivity is predicted to be wide (human, mouse, rat, and others), but it is advisable to verify reactivity in the specific sample system of interest, especially for non-mammalian species.
• As a rabbit polyclonal antibody, batch-to-batch consistency should be evaluated for critical quantitative assays. Including appropriate positive and negative controls (e.g., monomethyl peptide competition) can enhance data reliability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials and technical services. This anti-monoMethyl Histone H3-R2 antibody is ideal for epigenetic and chromatin research applications. Beyond this product, CamelBio can supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assist in sourcing rare-target raw materials to accelerate your IVD development from concept to clinic. Partner with CamelBio for reliable and scalable raw-material solutions.