MonoMethyl-Histone H3-R17 Rabbit Polyclonal Antibody (Q16695/P68431) for WB, IHC-P, IF/ICC, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

Histone H3 is a core component of the nucleosome, the fundamental unit of chromatin, which compacts genomic DNA and regulates access to transcription factors, DNA repair, and replication machinery. This structural and regulatory role is essential for chromosomal stability and gene expression. Related references: PMID:17194708

Arginine methylation of histone H3 is a key post-translational modification within the histone code. Specifically, monomethylation at arginine 17 (R17) is catalyzed by coactivator-associated arginine methyltransferase 1 (CARM1) and has been implicated in transcriptional activation and chromatin remodeling. Related references: PMID:15345777

Histone H3 is predominantly localized in the nucleus, where it assembles into nucleosome core particles along with other histone proteins. Related references: P68431

While canonical H3.1 is ubiquitously expressed, the human genome encodes several histone H3 variants with specialized functions; for instance, the testis-specific H3.1t variant (UniProt Q16695) is expressed predominantly in testicular cells. Related references: Q16695

Experimental Guidance and Technical Tips

• The immunogen is a synthetic monomethylated peptide corresponding to residues around R17 of human histone H3. For Western blot (WB), acid-extracted histones or whole‑cell lysates are recommended; a specific band at approximately 15 kDa should be expected.
• For immunohistochemistry (IHC-P), consider optimizing antigen retrieval conditions (e.g., heat‑mediated citrate buffer) to expose the epitope.
• In immunofluorescence (IF/ICC), use mild fixation (e.g., 4% paraformaldehyde) to preserve the monomethylation modification and avoid over‑crosslinking.
• This antibody is also suitable for ELISA‑based detection of monomethylated H3-R17 peptides; consider using a suitable blocking buffer and verifying the signal‑to‑noise ratio.
• Predicted to cross‑react with a wide range of species (Human, Mouse, Rat, others). Validate the antibody in the target species and sample type before large‑scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized one‑stop sourcing partner for IVD raw materials, offering diagnostic manufacturers and research institutes seamless access to high‑quality immunological reagents. This MonoMethyl‑Histone H3‑R17 rabbit polyclonal antibody exemplifies our commitment to providing validated, application‑ready antibodies for epigenetics research. Beyond individual antibodies, CamelBio supports your entire workflow with validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing of rare‑target raw materials—ensuring a reliable and efficient supply chain from concept to clinic.