MonoMethyl-Histone H3-K9 Rabbit mAb (Q16695/P68431) — Monoclonal Antibody for Epigenetic Research

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

Histone H3 is a core component of nucleosomes, which wrap and compact DNA into chromatin, restricting DNA accessibility to transcriptional, repair, and replication machinery. Through extensive post-translational modifications (the “histone code”), histone H3 plays a central role in regulating transcription, DNA repair, replication, and chromosomal stability.

Related references: UniProt Q16695, UniProt P68431

Subcellularly, histone H3 is localized to the nucleus and chromosomes. Two main variants are represented by the Swiss-Prot entries: H3.1t (Q16695) is primarily expressed in testicular cells, while the canonical H3.1 (P68431) is expressed in most tissues.

Related references: UniProt Q16695, UniProt P68431

Among the many modifications, mono-methylation at lysine 9 (H3K9me1) is a key mark associated with gene silencing and heterochromatin formation. This modification is dynamically regulated by methyltransferases and demethylases, influencing chromatin architecture and gene expression profiles.

Related references: UniProt P68431

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide specifically monomethylated at K9; consider using a non-methylated peptide or other methylation-state peptides as controls to confirm monospecificity.
• Validated applications include Western blot (WB), dot blot (DB), immunohistochemistry-paraffin (IHC-P), immunofluorescence/immunocytochemistry (IF/ICC), and ELISA. Optimal dilutions should be determined empirically.
• The antibody is predicted to cross-react with a wide range of species beyond human, mouse, and rat; validate in your specific sample system to ensure reactivity.
• For IHC-P and IF/ICC, appropriate antigen retrieval and permeabilization may enhance signal; consider testing different fixation protocols.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For your epigenetic or chromatin research needs, we offer validated monoclonal and polyclonal antibodies, optimized matched antibody pairs, and bulk ancillary reagents. Inquire about our rare-target raw-material sourcing capabilities, including highly specific mono-methylation state antibodies like this H3K9me1 mAb.