MonoMethyl-Histone H3-K9 (Q16695/P68431) Rabbit pAb for WB, ChIP-seq, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

• Histone H3 is a core component of the nucleosome, wrapping and compacting DNA into chromatin. This structural role is fundamental to transcription regulation, DNA repair, DNA replication, and chromosomal stability. Related references: UniProt Q16695, UniProt P68431
• The H3.1 and H3.1t variants (encoded by genes such as H3C1, H3C2, etc., and H3-4, respectively) share high sequence similarity and localize to the nucleus and chromosomes. Related references: UniProt Q16695, UniProt P68431
• H3.1t (Q16695) is specifically expressed in testicular cells, suggesting a specialized role in chromatin organization during spermatogenesis. Related references: UniProt Q16695
• Histones undergo a wide array of post-translational modifications, including methylation, acetylation, phosphorylation, and ubiquitination. Lysine 9 of histone H3 (H3K9) is a critical residue for methylation, with mono-, di-, and trimethylation states each having distinct biological outcomes. This antibody was raised against a synthetic monomethylated peptide around K9, enabling specific detection of H3K9me1. Related references: UniProt Q16695, UniProt P68431
• The dynamic regulation of H3K9 methylation is involved in heterochromatin formation, transcriptional repression, and epigenetic inheritance. Monomethylation (H3K9me1) is often associated with euchromatic regions and gene activation, though its functional role can vary by genomic context. Related references: UniProt Q16695, UniProt P68431

Experimental Guidance and Technical Tips

• For Western blotting (WB), we recommend using nuclear-enriched lysates to enhance detection of histone H3. The predicted molecular weight is ~15 kDa; adjust gel acrylamide percentage and transfer conditions accordingly. Always include appropriate molecular weight markers.
• When using this antibody for immunoprecipitation-based applications like ChIP and ChIP-seq, verify specificity by including negative controls (e.g., non-methylated peptide, or cells/tissue lacking the modification) and positive controls (e.g., cells known to harbor H3K9me1). Crosslink optimization and fragmentation efficiency are critical.
• For immunohistochemistry (IHC-P) or immunofluorescence (IF/ICC), consider antigen retrieval methods and permeabilization steps to expose nuclear epitopes. Validate staining in known positive and negative tissues.
• Given the predicted wide cross-reactivity, the antibody is expected to recognize H3K9me1 in human, mouse, rat, and other species. We still advise confirming reactivity in your specific sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw material procurement, connecting diagnostic manufacturers, laboratories, and research institutes with high-quality antibodies and ancillary reagents. This monoMethyl-Histone H3-K9 Rabbit pAb is ideal for epigenetic research and assay development, supporting applications from basic research to clinical translation. In addition to this product, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your IVD development pipeline.