MonoMethyl-Histone H3-K79 Rabbit Polyclonal Antibody (Q16695/P68431) for WB, IHC, ChIP

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

Histone H3 is a core component of the nucleosome, the fundamental repeating unit of chromatin. Together with H2A, H2B, and H4, it packages genomic DNA into compact structures, thereby regulating access for DNA-templated processes such as transcription, replication, repair, and chromosomal stability. The dynamic modulation of chromatin architecture is achieved through a complex array of post-translational modifications on histone tails and globular domains, collectively referred to as the "histone code". Related references: UniProt Q16695, UniProt P68431

Histone H3 localizes primarily to the nucleus and is associated with chromosomes throughout the cell cycle. Related references: UniProt Q16695, UniProt P68431

While canonical histone H3 variants are ubiquitously expressed, certain histone H3 isoforms exhibit tissue-specific patterns. For example, histone H3.1t (H3t), encoded by H3-4 (HIST3H3), is predominantly expressed in testicular cells. Related references: UniProt Q16695

Histone H3 undergoes extensive post-translational modifications, including methylation, acetylation, phosphorylation, citrullination, and others, at various residues. Among these, methylation at lysine 79 (K79) is a conserved epigenetic mark, with mono-, di-, and tri-methylation states each associated with distinct chromatin functions. This polyclonal antibody has been developed using a synthetic monomethylated peptide around K79 to specifically detect the K79me1 modification.

Experimental Guidance and Technical Tips

• The immunogen was designed as a synthetic peptide corresponding to the region surrounding lysine 79 of human histone H3, bearing a monomethyl group at K79. This ensures specific reactivity toward the monomethylated form; cross-reactivity with unmethylated or higher methylation states should be assessed in the experimental context.
• For Western blotting (WB), consider using acid-extracted histones or chromatin-enriched nuclear fractions to enhance detection, with typical protein migration observed at ~15 kDa.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), validate the antibody under appropriate fixation and antigen retrieval conditions for the tissue or cell type of interest.
• For chromatin immunoprecipitation (ChIP) and ChIP-sequencing (ChIP-seq), optimize shearing and antibody amount for the specific chromatin input; inclusion of positive and negative genomic loci for H3K79me1 is recommended.
• In ELISA-based assays, the antibody can be employed to confirm peptide specificity; validate performance with a panel of methylated and unmethylated peptides.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with integrated access to high-quality IVD raw materials, technical support, and consulting services. This anti-monoMethyl-Histone H3-K79 antibody is relevant for epigenetics research and assay development. Beyond individual antibody sourcing, CamelBio offers one-stop procurement solutions including validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and assistance in securing rare-target raw materials to streamline your workflow from concept to clinic.