MonoMethyl-Histone H3-K79 Rabbit Monoclonal Antibody (Q16695/P68431) – WB, ChIP, IHC-P, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

• Histone H3 is a core component of nucleosomes, essential for DNA packaging into chromatin. It plays a pivotal role in transcription regulation, DNA repair, DNA replication, and maintenance of chromosomal stability. Post-translational modifications (PTMs) on histone H3, such as methylation, acetylation, phosphorylation, and ubiquitylation, form a dynamic "histone code" that modulates chromatin accessibility and gene expression.
  Related references: UniProt Q16695, UniProt P68431

• Subcellularly, histone H3 localizes to the nucleus and chromosomes. Among the H3 variants, H3.1t (Q16695) is expressed primarily in testicular cells, while H3.1 (P68431) is encoded by a cluster of replication-dependent genes and represents the canonical H3 somatic form.
  Related references: UniProt Q16695 (tissue specificity), UniProt P68431 (subcellular location)

• Lysine 79 (K79) of histone H3 is a well‑characterized residue that undergoes mono‑, di‑, and trimethylation. These methylation states exert distinct regulatory influences on chromatin structure and function, with K79me1 being implicated in processes such as transcription elongation and telomeric silencing.
  Related references: UniProt Q16695, UniProt P68431 (annotated modifications at K79)

Experimental Guidance and Technical Tips

• Immunogen Design: The immunogen is a synthetic monomethylated peptide surrounding K79 of human histone H3. For applications requiring high specificity to K79me1, consider using unmodified histone H3 or di‑/trimethylated K79 peptides as negative controls to confirm antibody selectivity.
• Western Blotting (WB): A band at approximately 15 kDa is expected in whole‑cell lysates from human, mouse, or rat samples. Optimize antibody dilution and incubation conditions for your specific sample type.
• Chromatin Immunoprecipitation (ChIP/ChIP‑seq): This antibody is suitable for native or cross‑linked ChIP. Validate enrichment efficiency by qPCR or sequencing with appropriate positive and negative genomic loci.
• ELISA and DB: Direct binding assays can be used; ensure the coating peptide maintains the correct methylation status.
• Cross‑Reactivity: Predicted wide cross‑reactivity suggests conservation of the K79me1 epitope across species. Verify performance in less common species with pilot experiments.
• Store and handle as recommended; shipped on ice bags.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio is dedicated to empowering diagnostic manufacturers, core labs, and research institutes with end‑to‑end access to IVD raw materials. From monoclonal antibodies and recombinant proteins to bulk ancillary reagents, our supply network and technical support streamline procurement from early‑stage R&D to clinical application. Whether you require validated antibody pairs for immunoassay development or rare‑target sourcing, we provide reliable, integrated solutions. For epigenetics and chromatin biology, this high‑quality anti‑MonoMethyl‑Histone H3 (K79) rabbit mAb complements our portfolio of histone modification tools.