MonoMethyl-Histone H3-K4 Rabbit pAb (Q16695/P68431) — Validated for WB, IHC-P, ChIP & More

Core Product Specifications and Parameters

Biological Background: Histone H3 (MonoMethyl-K4) Function and Localization

• Core nucleosome component – Histone H3 is a fundamental building block of the nucleosome, wrapping DNA into chromatin and thereby regulating access for transcription, replication, repair, and chromosomal stability. Post‑translational modifications (the “histone code”) control these dynamic processes.

  Related references: UniProt Q16695, UniProt P68431

• Subcellular localization – Histone H3 resides in the nucleus and is an integral component of chromosomes.

• H3K4me1 epigenetic mark – Monomethylation of lysine 4 on histone H3 (H3K4me1) is a conserved post‑translational modification enriched at enhancers and poised promoters. It plays a critical role in regulating gene activation and is part of the intricate histone code that governs chromatin states.

  Related references: PMID:16267050, PMID:16457588, PMID:17194708

• Tissue expression – Canonical histone H3 (P68431) is ubiquitous, while the testis‑specific variant H3.1t (Q16695) is expressed primarily in testicular cells, contributing to tissue‑specific chromatin organization.

  Related references: UniProt Q16695 tissue specificity

• Immunogen design – The antibody was generated using a synthetic monomethylated peptide corresponding to residues around Lys4 of human histone H3, ensuring high specificity toward the H3K4me1 modification.

Experimental Guidance and Technical Tips

• In Western blot, expect a single band migrating at approximately 15 kDa; use histone extracts or acid‑extracted nuclear fractions.
• For ChIP and ChIP‑seq, optimize cross‑linking, fragmentation, and antibody concentration; include input and positive/negative genomic regions (e.g., active enhancers vs. silent loci).
• For IHC‑P and IF/ICC, validate staining with tissues or cells known to exhibit H3K4me1 enrichment; include peptide competition controls using methylated and non‑methylated peptides.
• Consider ELISA‑based assays for high‑throughput screening or antibody titer determination.
• Because the antibody is predicted to recognize human, mouse, rat, and other species, verify cross‑reactivity in your specific experimental models.
• Handle and store the antibody according to the recommended conditions (shipped on ice bag) to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a streamlined, one‑stop sourcing platform for IVD raw materials. Our portfolio includes validated monoclonal and polyclonal antibodies, antigen‑matched pairs, bulk ancillary reagents, and rare‑target raw materials. For epigenetics and chromatin research, we offer high‑quality antibodies like this H3K4me1 pAb to support assay development, validation, and scalable supply—all backed by responsive technical consultation.