MonoMethyl-Histone H3-K4 Rabbit Monoclonal Antibody (Q16695/P68431) for WB, ChIP, IF/ICC, and Epigenetic Research

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

Histone H3 is a core component of the nucleosome, which wraps and compacts DNA into chromatin. This compaction limits DNA accessibility to the cellular machinery that requires DNA as a template, thereby playing a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is finely tuned through a complex set of post-translational modifications on histones, known as the histone code, and by nucleosome remodeling.

Histone H3 localizes to the nucleus and is an integral part of chromosomes. Two variants are relevant here: the canonical histone H3.1 (encoded by multiple genes such as H3C1, H3C2, etc., SWISS P68431) is ubiquitously expressed, whereas histone H3.1t (SWISS Q16695) is testis-specific. Both share the same fundamental role in chromatin structure.

Lysine 4 of histone H3 (H3K4) is a major site for diverse post-translational modifications, including mono-, di-, and trimethylation, acetylation, and others. Monomethylation of H3K4 (H3K4me1) is typically associated with enhancer regions and active gene transcription. This antibody was raised against a monomethylated peptide surrounding H3K4, providing specificity for this modification state.

Related references: PMID:16267050, PMID:16457588, PMID:17194708.

Experimental Guidance and Technical Tips

• This antibody is specific for monomethylated H3K4. For optimal results, include positive and negative control samples (e.g., peptides with unmodified, mono-, di-, or tri-methylated K4) to confirm specificity.
• Western Blot (WB): Use nuclear extracts or whole cell lysates. A band at approximately 16 kDa is expected. Determine the optimal primary antibody dilution empirically (e.g., starting range 1:500–1:2000) and pair with an appropriate HRP-conjugated secondary antibody.
• Chromatin Immunoprecipitation (ChIP): Suitable for ChIP. Cross-link chromatin, shear, and immunoprecipitate with the antibody. Validate enrichment by qPCR at known H3K4me1 target loci.
• Immunofluorescence (IF/ICC): Fix and permeabilize cells appropriately. Expect exclusive nuclear staining. Counterstain with DAPI or similar to confirm localization.
• IHC-P: Antigen retrieval may be required; optimize conditions for each tissue type.
• Dot Blot (DB) and ELISA: Ensure peptide concentration and blocking conditions are optimized to minimize non-specific binding.
• Cross-reactivity: This antibody recognizes a wide range of species including plants (Arabidopsis, rice, wheat). For plant samples, adjust extraction protocols due to the rigid cell wall and optimize detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with streamlined access to high-quality IVD raw materials. This MonoMethyl-Histone H3-K4 Rabbit mAb supports cutting-edge epigenetic and chromatin biology research. Beyond validated antibody pairs and optimized monoclonal antibodies, CamelBio facilitates bulk procurement of ancillary reagents and sourcing of rare‑target raw materials, accelerating your journey from concept to clinic.