MonoMethyl-Histone H3-K4 Rabbit mAb (Q16695/P68431) for WB, IF/ICC, CUT&Tag

Core Product Specifications and Parameters

Biological Background: Histone H3 (Q16695/P68431) Function and Localization

Histone H3 is a core component of the nucleosome, wrapping DNA around histone octamers to compact DNA into chromatin. This structure limits DNA accessibility to cellular machineries, thereby playing a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
Related references:
UniProt entry for Q16695/P68431

Post-translational modifications (PTMs) on histone tails, such as methylation, acetylation, and phosphorylation, constitute the “histone code” that governs chromatin dynamics and gene expression. Methylation at lysine residues, including monomethylation, serves as a recognition site for effector proteins, modulating transcriptional states.
Related references:
PMID:16267050
PMID:17194708
PMID:16457588

Histone H3 localizes to the nucleus and chromosomes, where it participates in the dynamic regulation of chromatin structure. The canonical Histone H3.1 (P68431) is broadly expressed in somatic cells, while the testis-specific isoform Histone H3.1t (Q16695) is confined to testicular cells, highlighting tissue-specific roles.
Related references:
UniProt entry for P68431

The highly conserved nature of Histone H3 across species underlies its cross-reactivity, making it a valuable target for epigenetic studies and diagnostic applications in human, mouse, rat, and other organisms.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic monomethylated peptide corresponding to the K4 region of Histone H3; the antibody may specifically recognize the monomethylated epitope across species due to sequence conservation.
• For Western blot (WB), a band around 15 kDa is expected; consider optimization of blocking and antibody dilution for your specific sample type.
• In IF/ICC, nuclear chromatin staining is anticipated; evaluate fixation and permeabilization conditions to ensure epitope accessibility.
• For CUT&Tag, this antibody can be used to profile H3K4 monomethylation genome-wide; it is advisable to validate with positive and negative control genomic loci.
• Cross-reactivity is predicted across a wide range of species (Human, Mouse, Rat, Other); however, potential cross-reactivity with other methylation states (e.g., dimethyl, trimethyl at K4) should be assessed in the experimental system.
• Shipment on ice bag is recommended to maintain antibody integrity; store according to manufacturer guidelines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a one-stop sourcing solution for IVD raw materials, including optimized monoclonal antibodies like the MonoMethyl-Histone H3-K4 antibody. Leveraging expertise in epigenetic targets and PTM-specific reagents, CamelBio supports the development of robust diagnostic assays through reliable supply of validated antibody pairs, bulk ancillary reagents, and rare target raw materials. From concept to clinic, CamelBio bridges the gap with tailored technical consulting and efficient sourcing.