MonoMethyl-Histone H3-K36 Rabbit Polyclonal Antibody (Q16695/P68431) - WB, ChIP, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

Histone H3 is a core component of the nucleosome, wrapping and compacting DNA to limit accessibility for transcription, replication, and repair. The complex set of post-translational modifications on histones, commonly referred to as the histone code, regulates chromatin structure and function.

Histone H3 localizes to the nucleus and is a primary constituent of chromosomes.

The canonical histone H3.1 (encoded by multiple human gene clusters, SWISS P68431) is expressed broadly, while the testis-specific variant H3.1t (H3-4, SWISS Q16695) is predominantly found in testicular cells.

Lysine 37 of histone H3 (often denoted K36 in mature histone nomenclature) is a well-characterized site for methylation. Mono-, di-, and tri-methylation of this residue are catalyzed by methyltransferases such as SETD2 and NSD family members, playing critical roles in transcriptional regulation, DNA repair, and alternative splicing. Related references: PMID:15983376, PMID:16185088, PMID:16267050

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N‑terminal tail (aa 1–100) of human histone H3, which encompasses the K36 residue. This design may provide good specificity for the mono‑methylated form when validated by blocking peptide assays.
• For Western blotting, use nuclear or histone‑enriched extracts from cells. A band near 15 kDa is expected; confirm specificity by using peptide competition or comparing signals in methylation‑modulated cell lysates.
• In ChIP and ChIP‑seq experiments, antibody specificity under crosslinking conditions should be verified with positive and negative loci known to harbor H3K36me1 marks. Consider spike‑in controls for normalization.
• Cross‑reactivity with mouse, rat, and other species is predicted based on sequence homology; validate in the relevant sample system if using non‑human species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers IVD developers with streamlined access to validated raw materials, including methylation‑specific antibodies like this anti‑H3K36me1 product. Our platform offers a comprehensive portfolio of optimized monoclonal and polyclonal antibodies, antibody pairs, bulk ancillary reagents, and rare‑target sourcing to support epigenetic diagnostics and research. With one‑stop procurement from concept to clinic, CamelBio helps diagnostic manufacturers and labs accelerate assay development and secure consistent, research‑grade supplies.