MonoMethyl-Histone H3-K36 Rabbit mAb (Q16695/P68431) for WB, DB, IHC-P, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

Histone H3 is a core component of the nucleosome, which wraps and compacts DNA into chromatin. This compaction limits DNA accessibility to the cellular machineries that require DNA as a template. Consequently, histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, collectively known as the histone code, and through nucleosome remodeling. Related references: UniProt Q16695, UniProt P68431

Multiple genes encode histone H3 variants. The product's remarks and aliases include H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3C10, H3C11, H3C12, and HIST1H3A, reflecting the various replication-dependent histone H3.1 genes. Additionally, the testis-specific variant H3.1t (H3t, encoded by H3-4 / HIST3H3) is represented by SWISS accession Q16695. These variants are highly similar in sequence. Related references: UniProt Q16695, UniProt P68431

Subcellularly, histone H3 is localized to the nucleus and is a major component of chromosomes. Its presence is ubiquitous in nucleosomes across the genome. Related references: UniProt Q16695, UniProt P68431

Post-translational modifications of histone H3, including methylation, acetylation, phosphorylation, and others, fine-tune chromatin structure and function. MonoMethylation at lysine 36 (K36) is one such modification. This antibody specifically detects the monomethylated form of K36 and was generated using a synthetic monomethylated peptide immunogen targeting this residue. Related references: UniProt Q16695, UniProt P68431

Experimental Guidance and Technical Tips

• The predicted protein weight of histone H3 is approximately 15 kDa. However, due to its highly basic nature, histone H3 often migrates at a slightly higher apparent molecular weight on SDS-PAGE. Verify the specific migration pattern in your sample system.
• This antibody was raised against a synthetic monomethylated peptide around K36. It is expected to recognize the monomethylated form specifically over unmodified or other methylated states. Confirm specificity using peptide competition assays or by comparing signals from cell lysates treated with methylation-modifying agents.
• For Western blot (WB) and dot blot (DB), consider using histone extraction protocols that enrich for nuclear proteins to improve detection sensitivity.
• In immunohistochemistry (IHC-P), antigen retrieval conditions (e.g., citrate buffer pH 6.0 or EDTA buffer pH 9.0) may need to be optimized. Include known positive and negative control tissues to evaluate staining specificity.
• ELISA can be used for high-throughput screening or titration experiments. Pair this antibody with a suitable anti-rabbit secondary antibody conjugated to HRP or another reporter enzyme for detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with streamlined access to a comprehensive portfolio of IVD raw materials. For epigenetic research and assay development, we supply high-quality antibodies like this MonoMethyl-Histone H3-K36 Rabbit mAb, along with validated antibody pairs, optimized monoclonal and polyclonal antibodies, blocking buffers, and bulk ancillary reagents. Our team also offers sourcing solutions for rare modification-specific antibodies and custom recombinant protein production, helping you accelerate your diagnostic product pipeline from concept to clinic.