MonoMethyl-Histone H3-K36 (Q16695 / P68431) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA, ChIP-seq

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

Histone H3 is a core component of the nucleosome, which wraps and compacts DNA into chromatin. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called the histone code, and nucleosome remodeling.

Histone H3 localizes to the nucleus and chromosomes, consistent with its fundamental role in chromatin structure.

The UniProt accessions Q16695 and P68431 correspond to histone H3.1t (testis-specific) and H3.1 (canonical), respectively. H3.1t (Q16695) is expressed in testicular cells, whereas H3.1 (P68431) is broadly expressed as a replication‑dependent histone.

Histone H3 undergoes extensive post‑translational modifications at numerous residues, including methylation, acetylation, phosphorylation, and others. Notably, monomethylation at lysine 36 (corresponding to residue 37 of the full‑length protein) has been identified in proteomic studies. This modification is one of many that contribute to the histone code and regulate chromatin function.

Related references: PMID:15983376, PMID:16185088, PMID:16267050

Experimental Guidance and Technical Tips

• For Western blot (WB), a band around 15 kDa is expected. Consider using nuclear extracts and validating specificity with a monomethylated peptide competition assay.
• In immunofluorescence (IF/ICC), nuclear staining should be observed. Fixation and permeabilization conditions may require optimization; consider testing paraformaldehyde versus methanol fixation.
• For ChIP‑seq, this antibody can be used to map H3K36me1 distribution across the genome. Include appropriate controls such as input DNA and a non‑immune IgG. H3K36me1 is typically enriched in gene bodies of actively transcribed genes.
• The antibody is predicted to cross‑react widely across species based on sequence conservation. Validation in non‑human, mouse, or rat samples is recommended before use.
• Combine with other histone modification antibodies to study epigenetic crosstalk. Always validate in the relevant sample system and consider lot‑to‑lot consistency for quantitative applications.

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