MonoMethyl-Histone H3-K27 Rabbit mAb (Q16695/P68431) – Methylated Histone H3 Antibody for WB, IF/ICC, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

• Histone H3 is a core component of the nucleosome, wrapping and compacting DNA into chromatin. This structure limits DNA accessibility to cellular machineries, thereby playing a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called the histone code, and nucleosome remodeling. Related references: UniProt Q16695, UniProt P68431

• Histone H3 localizes to the nucleus and associates with chromosomes. Related references: UniProt Q16695, UniProt P68431

• The canonical histone H3.1 isoforms (encoded by multiple genes) are generally expressed in dividing cells, while the H3.1t variant (Q16695) is specifically expressed in testicular cells. Related references: UniProt Q16695

• Histone H3 undergoes extensive post-translational modifications, including methylation, acetylation, phosphorylation, and citrullination at specific residues. Lysine 27 (K27) is a known modification site; its monomethylated state (H3K27me1) is associated with gene regulation. Related references: PMID:16567635

Experimental Guidance and Technical Tips

• The immunogen is a synthetic monomethylated peptide around K27, suggesting high specificity for the H3K27me1 mark. When using the antibody, consider including methylated and unmodified peptide controls in dot blot or ELISA assays to validate specificity.
• For Western blotting (WB), histone-enriched acid extracts or total nuclear lysates are recommended. A typical loading amount of 10–20 µg of nuclear extract may be used, but optimization in the relevant sample system is advised.
• For immunofluorescence/immunocytochemistry (IF/ICC), permeabilization with Triton X-100 or similar detergent is necessary to allow nuclear staining. Co-staining with a total H3 antibody can help normalize methylation signals.
• Cross-reactivity is predicted across a wide range of species due to sequence conservation; however, always validate the antibody in the target species or tissue if not previously tested.
• This antibody is applicable for epigenetic research focusing on histone modifications and chromatin dynamics; consider its use in ChIP-like applications after careful validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with a comprehensive one-stop portfolio of IVD raw materials, technical services, and consulting—guiding projects from concept to clinic. For histone modification research and epigenetic assay development, we can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies (including methylation-specific clones), bulk ancillary reagents, and rare-target raw-material sourcing. Whether you need this anti-H3K27me1 rabbit mAb or customized solutions for chromatin biology, CamelBio is your reliable partner for quality, consistency, and rapid global delivery.