MonoMethyl-Histone H3-K27 (H3K27me1) Rabbit Polyclonal Antibody (Q16695/P68431) for WB, IHC, ChIP & ELISA

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

• Histone H3 is a core component of the nucleosome, serving as a scaffold for DNA packaging and playing a pivotal role in regulating transcription, DNA replication, and repair. Post-translational modifications (PTMs) of histone H3, including methylation at specific lysine residues, constitute a dynamic "histone code" that governs chromatin structure and gene expression. Related references: PMID:16267050, PMID:17194708
• Subcellularly, histone H3 is localized to the nucleus and associated with chromosomes in both interphase and mitotic cells.
• Tissue expression: The canonical histone H3.1 (encoded by multiple genes including H3C1, H3C2, etc.) is widely expressed in somatic tissues, while the testis-specific variant H3.1t (Q16695) shows restricted expression in testicular cells.
• Lysine 27 (K27) of histone H3 is a critical residue for post-translational modifications, including citrullination PMID:16567635. Monomethylation of H3K27 (H3K27me1) is one of the methylation states that influences chromatin architecture and transcription. This antibody specifically detects H3K27me1.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic monomethylated peptide around K27 of human histone H3. When using this antibody, consider performing peptide competition assays to confirm modification-specific binding.
• For Western blot (WB) analysis, a band at approximately 15 kDa corresponding to histone H3 is expected. Use of appropriate loading controls and histone extraction protocols may improve detection.
• In immunoprecipitation-based applications such as ChIP, ensure chromatin is sufficiently fragmented and validate antibody specificity using positive and negative genomic loci (e.g., regions with known H3K27me1 enrichment).
• Compatibility with multiple species (human, mouse, rat, and others) is predicted based on sequence homology; however, it is advisable to validate cross-reactivity in the relevant species and experimental system.
• Optimal working dilutions for ELISA, dot blot (DB), and immunohistochemistry should be determined empirically by the end user.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, bridging diagnostic manufacturers and research labs with a vast portfolio of high-quality antibodies and ancillary reagents. For projects targeting histone modifications such as H3K27 monomethylation, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk raw materials to support your assay development from concept to clinic. Let CamelBio streamline your sourcing needs, whether you require routine IVD components or specialized rare-target antibodies.