MonoMethyl-Histone H2B-K5 Rabbit Polyclonal Antibody (P62807/Q16778) for WB, IF/ICC, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H2B Function and Localization

Histone H2B is a core component of the nucleosome, wrapping and compacting DNA into chromatin. This compaction limits DNA accessibility and plays a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. H2B exists as several isoforms encoded by distinct genes, with UniProt accessions P62807 (Histone H2B type 1-C/E/F/G/I) and Q16778 (Histone H2B type 2-E) representing major variants.

Beyond its structural role, H2B has broad antibacterial activity and may contribute to the antimicrobial barrier of the colonic epithelium and the bactericidal activity of amniotic fluid.

H2B is extensively post-translationally modified, contributing to the "histone code" that regulates chromatin dynamics. Modifications include acetylation, methylation, phosphorylation, crotonylation, and ubiquitination on multiple lysine, serine, and arginine residues. The specific monomethylation at lysine 5 (K5me1) is one such mark that can influence gene expression and chromatin state. The antibody described here was raised against a synthetic monomethylated peptide corresponding to K5me1 of human H2B, providing a tool for epigenetic research.

Histone H2B localizes to the nucleus and chromosomes, consistent with its nucleosomal function.

Related references: Histone modifications such as acetylation and methylation have been extensively characterized PMID:16627869. Additional studies have identified novel acylations including crotonylation PMID:21925322 and lactylation PMID:31645732 on H2B lysines.

Experimental Guidance and Technical Tips

• For Western blotting (WB), use nuclear extracts to enrich histone proteins. The signal for monomethyl-H2B-K5 may be low; consider using peptide competition assays to confirm specificity.
• In immunofluorescence (IF/ICC), ensure proper cell permeabilization and consider chromatin pre-extraction to improve antibody accessibility to histone targets.
• For ELISA-based assays, coat plates with the monomethylated peptide or histone extracts and validate with the antibody.
• The antibody has predicted wide cross-reactivity (human, mouse, rat, and others); nonetheless, verify reactivity in your specific sample system with appropriate controls.
• As with any post-translational modification-specific antibody, lot-to-lot variability may occur; include known positive and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents covering a broad range of targets. For rare or historically difficult-to-source targets like site-specific histone modifications, CamelBio leverages its extensive network to offer customized sourcing solutions. Our commitment is to streamline your development pipeline with reliable, high-quality raw materials and expert support, enabling your next breakthrough in epigenetic IVD assay development.