MonoMethyl-H2B-E105 Rabbit Polyclonal Antibody (P33778) – Histone H2B type 1-B Monomethyl Specific for WB, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H2B type 1-B Function and Localization

Histone H2B type 1-B (gene symbol: H2BC3; also known as H2BFF, HIST1H2BB, H2B.1, H2B/f) is a core component of the nucleosome, the fundamental repeating unit of chromatin. Nucleosomes wrap and compact DNA, limiting its accessibility to the cellular machineries that require DNA as a template. Consequently, histone H2B plays a central role in the regulation of transcription, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is dynamically regulated through a complex set of post-translational modifications (PTMs) on histones, collectively referred to as the histone code, as well as through nucleosome remodeling.

Histone H2B is predominantly localized in the nucleus and on chromosomes, where it interacts with other core histones to form the nucleosome. It is subject to an extensive array of post-translational modifications, including acetylation, methylation, phosphorylation, ADP-ribosylation, ubiquitination, and various acylations. For example, lysine residues such as K6, K13, K16, K21, and K86 can be acetylated, while others like K47 and K109 can be methylated. Many of these modifications have been mapped and functionally characterized in the context of gene expression and chromatin dynamics.

This antibody was raised against a synthetic monomethylated peptide corresponding to a region around glutamic acid 105 (E105) of human histone H2B (NP_066406.1). While monomethylation of glutamic acid is less common than methylation of lysine or arginine, it represents a potential regulatory mechanism. The antibody is intended to recognize the monomethylated form of H2B at E105, enabling the study of this specific modification in epigenetic research.

Related references:

• Function: UniProt P33778
• Modifications: PMID:16283522, PMID:21925322, PMID:24681537

Experimental Guidance and Technical Tips

• The immunogen is a synthetic monomethylated peptide around E105 of human H2B; consider validating the antibody's specificity for the monomethylated form using appropriate controls, such as non-methylated peptide or histone extracts with known modification states.
• For Western blot, a band corresponding to approximately 14 kDa is expected; optimize protein extraction and detection protocols for histones to ensure efficient transfer and signal.
• Cross-reactivity is reported for human and mouse; if using in rodent models, verify performance in the relevant tissue or cell line.
• ELISA applications may be developed by coating with the immunogen peptide or histone extracts; consider the sensitive detection of methylation levels.

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CamelBio is your dedicated one-stop partner for IVD raw material sourcing, offering diagnostic manufacturers and research labs a seamless bridge from concept to clinic. Our epigenetic portfolio includes a range of histone modification antibodies like this MonoMethyl-H2B-E105 pAb, along with validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. We streamline your development workflow with reliable, high-quality raw materials and technical support, helping you advance your diagnostic and research projects efficiently.