Monkeypox virus (MPXV) E8L Rabbit mAb - Q8V4Y0

Core Product Specifications and Parameters

Biological Background: Cell surface-binding protein OPG105 Function and Localization

• Cell surface-binding protein OPG105, also known as carbonic anhydrase homolog, is encoded by the OPG105 gene of Monkeypox virus (MPXV) and serves as a viral envelope protein.
• OPG105 binds to chondroitin sulfate on the host cell surface, mediating virion attachment and facilitating viral entry into target cells.
• The protein localizes to the virion membrane and contains a transmembrane helix, anchoring it in the viral envelope.
• Its structure includes disulfide bonds, which likely stabilize the extracellular domain involved in host binding.
• Key functional keywords associated with OPG105 include host-virus interaction, viral attachment to host cell, and virus entry into host cell.
• The calculated molecular weight of OPG105 is approximately 35.3 kDa, consistent with its detection as a ~35 kDa protein in viral preparations.

Experimental Guidance and Technical Tips

• The immunogen for this antibody is a recombinant fusion protein spanning amino acids 1–275 of the E8L protein, corresponding to the full-length OPG105; this design likely supports recognition of native MPXV E8L.
• For Western blot analysis, consider using MPXV-infected cell lysates or recombinant OPG105 as positive controls, and validate detection of the expected ~35 kDa band under reducing conditions.
• In ELISA applications, the antibody may be employed as either a capture or detection antibody; optimize coating concentration and blocking conditions to achieve optimal signal-to-noise ratios.
• As the antibody is specific to Monkeypox virus, cross-reactivity with other orthopoxviruses should be experimentally tested if broader detection is desired.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research organizations with reliable access to validated IVD raw materials, including this anti-MPXV E8L rabbit monoclonal antibody. In addition to antibody pairs and bulk reagents, we offer technical services and consulting to support your assay development from concept to clinic. Whether you require rare-target sourcing or scalable ancillary materials, CamelBio is your dedicated partner for infectious disease diagnostics.