MON1A Rabbit Polyclonal Antibody for WB and ELISA - Q86VX9

Core Product Specifications and Parameters

Biological Background: MON1A Function and Localization

• MON1A (SAND1) is a vacuolar fusion protein homolog A that plays an important role in membrane trafficking through the secretory apparatus, but not in endocytic trafficking to lysosomes (By similarity).
• As a guanine exchange factor (GEF) for RAB7, MON1A works with CCZ1 to promote the exchange of GDP to GTP, converting RAB7 from an inactive to an active state. Related references: PMID:23084991
• The MON1A gene undergoes alternative splicing, producing multiple isoforms with potentially distinct functions.
• Post-translational modifications include phosphorylation, as documented in large-scale phosphoproteomic analyses.
• The three-dimensional structure of MON1A has been determined, aiding in understanding its GEF activity.
• MON1A is included in reference proteome sets and has been identified in various proteomics screens, suggesting constitutive expression.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal 1-250 residues of human MON1A, which contains functional domains; this region is expected to generate an antibody capable of detecting full-length and potentially some truncated variants.
• For Western blotting, prepare samples under reducing conditions and run alongside molecular weight markers; the expected band is around 73 kDa. Optimize antibody dilution and exposure times for your specific sample type.
• For ELISA, titrate the antibody to determine optimal working concentrations for direct or indirect formats; verify specificity with appropriate negative controls.
• Since the antibody is raised against human MON1A and shows human reactivity, cross-reactivity with other species should be tested if needed; sequence alignment can help predict potential reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined sourcing platform for diagnostic raw materials, from antibodies to ancillary reagents. For researchers investigating membrane trafficking and RAB7 signaling, our portfolio includes validated MON1A antibodies and related tools to accelerate assay development. Whether you need bulk oligoclonal antibodies or customized sourcing of rare targets, CamelBio supports your journey from concept to clinic.