MOCS3 Rabbit Polyclonal Antibody for WB and ELISA - O95396

Core Product Specifications and Parameters

Biological Background: MOCS3 Function and Localization

• MOCS3 (UBA4) is an adenylyltransferase and sulfurtransferase that plays a central role in 2-thiolation of tRNA wobble positions and molybdenum cofactor biosynthesis, acting on cytosolic tRNA(Lys), tRNA(Glu), and tRNA(Gln). Related references: PMID:19017811, PMID:22453920, PMID:30817134

• It catalyzes the C-terminal thiocarboxylation of the sulfur carriers URM1 and MOCS2A via a two-step mechanism: initial adenylation (-COAMP) followed by persulfide sulfur transfer to form -COSH. Related references: PMID:15073332, PMID:19017811, PMID:22453920

• The reaction depends on hydrogen sulfide generated from a persulfide intermediate, with NFS1 serving as the primary sulfur donor for thiocarboxylation. Related references: PMID:18650437, PMID:22453920

• MOCS3 is predominantly localized in the cytoplasm/cytosol, consistent with its role in cytosolic tRNA modification and molybdenum cofactor biosynthesis.

• It is a multifunctional enzyme with both nucleotidyltransferase and transferase activities, featuring ATP- and zinc-binding domains, and is classified under the reference proteome.

• Alternative names include molybdenum cofactor synthesis protein 3 and molybdopterin synthase sulfurylase, reflecting its dual roles in sulfur transfer.

Experimental Guidance and Technical Tips

• For Western blot, the expected band is around 50 kDa (predicted MW: ~49.7 kDa). Validate detection in relevant human or mouse cell lysates under reducing conditions.
• ELISA assays may benefit from coating with the recombinant immunogen; ensure the antibody recognizes the native epitope in your sample system.
• As a polyclonal antibody, lot-to-lot variability should be assessed; consider pre-testing each lot for critical applications.
• Cross-reactivity with human and mouse is indicated; validate performance in species-matched tissues to confirm specificity.

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