MNX1/HB9/HLXB9 Rabbit Monoclonal Antibody for WB and ELISA - P50219

Core Product Specifications and Parameters

Biological Background: MNX1/HB9/HLXB9 Function and Localization

• MNX1, also known as Homeobox protein HB9 (HLXB9), is a transcription factor that belongs to the homeobox family, playing critical roles in motor neuron specification and pancreas development.
• It binds to regulatory elements of target genes, such as the visual system homeobox CHX10, and negatively modulates transcription.
• In motor neurons, MNX1 collaborates with LIM domain transcription factor LMO4 to establish motor neuron identity and represses interneuron genes by interfering with the ISL1-LHX3 complex.
• MNX1 is also involved in pancreatic development and cell fate specification.
• The protein is localized to the nucleus, consistent with its function as a DNA-binding transcription factor.
• MNX1 undergoes post-translational modifications, including acetylation and phosphorylation, and exists in multiple isoforms due to alternative splicing.
• Disease-associated variants of MNX1 have been identified, linking it to certain genetic disorders (keyword: Disease variant).
• It is expressed in lymphoid and pancreatic tissues, indicating tissue-specific regulatory roles.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within amino acids 250-350 of human MNX1. This region may be conserved across species, supporting the observed cross-reactivity with mouse.
• For Western blot, the predicted molecular weight is 41 kDa; however, alternative splicing and post-translational modifications may result in multiple bands or shifts.
• Consider using a nuclear extract to enrich the target protein since MNX1 is a nuclear transcription factor.
• Validate antibody performance in your specific sample type and application with appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop partner for IVD raw material procurement, offering validated antibodies, optimized pairs, and bulk ancillary reagents tailored to your assay development needs. This MNX1 antibody supports research in neurobiology and developmental biology, and we can source complementary reagents such as paired antibodies, recombinant proteins, and ELISA components to streamline your workflow.