MMP9 Rabbit Polyclonal Antibody for WB, ELISA - P14780

Core Product Specifications and Parameters

Biological Background: MMP9 Function and Localization

• Matrix metalloproteinase-9 (MMP9), also known as 92 kDa gelatinase, gelatinase B (GELB), or CLG4B, is a zinc-dependent endopeptidase belonging to the matrix metalloproteinase family.
• Plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration.
  Related references: PMID:12879005 PMID:1480034 PMID:2551898
• Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments; degrades fibronectin but not laminin or Pz-peptide.
  Related references: PMID:1480034
• Cleaves KiSS1 at a Gly-|-Leu bond and NINJ1 to generate the secreted ninjurin-1 form.
  Related references: PMID:12879005 PMID:32883094
• Could play a role in bone osteoclastic resorption (By similarity).
• Subcellular location: Secreted into the extracellular space and extracellular matrix.
• Detected in neutrophils (at protein level) and produced by normal alveolar macrophages and granulocytes.
  Related references: PMID:7683678
• Features a signal peptide, propeptide, catalytic domain with zinc-binding site, hemopexin-like repeats, and undergoes zymogen activation. It is a glycoprotein that binds calcium and zinc.

Experimental Guidance and Technical Tips

• The immunogen maps to amino acids 538–707 within the C-terminal hemopexin-like domain of human MMP9, which may provide epitope specificity distinct from the catalytic domain.
• For Western blotting, anticipate a band around 78 kDa; run appropriate molecular weight markers and optimize blocking/detection conditions.
• For ELISA applications, consider using a recombinant MMP9 standard curve for quantification and validate antibody performance with your sample matrix.
• Cross-reactivity with mouse MMP9 is reported; verify performance in your experimental species and sample type if using mouse models.
• As a polyclonal antibody, lot-to-lot variability should be controlled by testing each new batch in your established assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing platform for IVD raw materials, connecting diagnostic manufacturers and research laboratories with high-quality reagents like this MMP9 rabbit polyclonal antibody. Beyond individual antibodies, we supply validated antibody pairs, bulk ancillary reagents, and rare target raw materials to streamline your assay development. Our integrated services support your journey from concept to clinic, with expert technical consultation available at every step.