MMP17 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9ULZ9

Core Product Specifications and Parameters

Biological Background: Matrix metalloproteinase-17 Function and Localization

• Matrix metalloproteinase-17 (MMP17), also known as MT4-MMP, is a membrane-type matrix metalloproteinase encoded by the MMP17 gene in humans.
• MMP17 is an endopeptidase that degrades extracellular matrix components such as fibrin. It may activate membrane-bound precursors of growth factors and inflammatory mediators, including tumor necrosis factor-alpha (TNF-α), by cleaving pro-TNF at the 74-Ala-|-Gln-75 site. However, it does not hydrolyze collagens I-V, gelatin, fibronectin, laminin, decorin, or alpha1-antitrypsin.
• The protein is anchored to the cell membrane via a GPI-anchor and is also secreted into the extracellular space and matrix.
• MMP17 is broadly expressed in brain, leukocytes, colon, ovary, testis, and breast cancer, as well as in many transformed and non-transformed cell types, indicating roles in normal physiology and tumor biology.
• Structurally, MMP17 features a signal peptide, a propeptide that is cleaved for activation, a catalytic domain with a zinc-binding motif, and a hemopexin-like repeat. It is synthesized as a zymogen and undergoes proteolytic activation at basic residues.
• Key functional and structural attributes include alternative splicing, GPI-anchoring, metal-binding, metalloprotease activity, and involvement in extracellular matrix remodeling, as highlighted in its UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 265–565, encompassing the catalytic and hemopexin-like domains, which may yield robust recognition of both pro- and active MMP17 forms.
• This antibody is indicated for western blotting (WB), immunohistochemistry on paraffin sections (IHC-P), and ELISA; for each application, consider optimizing antibody dilution, incubation conditions, and detection reagents in your specific sample matrix.
• As a rabbit polyclonal antibody, it likely contains a diverse pool of antigen-binding specificities, which may contribute to the reported cross-reactivity with human, mouse, and rat samples.
• Due to the GPI-anchored membrane localization of MMP17, efficient extraction for western blotting may require the use of appropriate lysis buffers containing detergents.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with integrated sourcing of IVD raw materials, technical services, and expert consultation from concept to clinic. This MMP17 rabbit polyclonal antibody exemplifies our commitment to providing validated antibody preparations for protease and cancer research. Beyond individual antibodies, we offer matched antibody pairs, bulk ancillary reagents, and access to rare targets—streamlining your assay development and manufacturing pipeline.