MLXIPL Rabbit pAb - Q9NP71

Core Product Specifications and Parameters

Biological Background: MLXIPL Function and Localization

• MLXIPL (also known as CHREBP, MONDOB, WS-bHLH, bHLHd14) is a class D basic helix-loop-helix protein encoded by the MLXIPL gene, with a molecular weight of approximately 93 kDa.
• Functions as a glucose-responsive transcription activator that regulates fatty acid synthesis and glycolysis, serving as a key determinant of systemic insulin sensitivity and glucose homeostasis.
• Important for the expression of fatty acid synthetic enzymes, including PC/Pcx, APOC4/Acl, ACACA/Acc1 and FASN/Fas, and for glucose-induced expression of L-type pyruvate kinase/PKLR (By similarity).
• Binds to the canonical and non-canonical E box DNA sequences 5'-CACGTG-3' and 5'-CACGCG-3', and may also act as a transcriptional repressor (By similarity).
• Localizes to both the cytoplasm and nucleus, consistent with its transcriptional regulatory role.
• Widely expressed, with high levels in heart, brain, placenta, skeletal muscle, and pancreas; also detected in fetal kidney, lung, liver, brain, and adult liver, as well as cerebral cortex and intestine.
• Keywords include alternative promoter usage, alternative splicing, phosphoprotein, DNA-binding, and association with Williams-Beuren syndrome, highlighting regulatory complexity.

Experimental Guidance and Technical Tips

• For Western blot, consider using nuclear or cytoplasmic extracts from human or mouse heart, liver, or pancreatic tissues as positive controls, as these are known to express MLXIPL.
• The predicted molecular weight is 93 kDa, but due to alternative splicing or post-translational modifications, multiple bands may be observed; validate under your experimental conditions.
• As a rabbit polyclonal antibody, lot-to-lot variability may occur; include appropriate negative and positive controls in each experiment and titrate the antibody to determine the optimal working dilution.
• For ELISA applications, validate the antibody with recombinant MLXIPL protein or known standards, and consider potential cross-reactivity with closely related family members.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing solution for IVD raw materials, supporting diagnostic manufacturers and researchers from assay development to clinical application. This high-quality MLXIPL polyclonal antibody is well-suited for metabolic disease research, particularly in diabetes and fatty acid metabolism studies. Beyond single antibodies, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing, streamlining your entire material procurement process.