MLH3 Rabbit pAb for WB and ELISA - Q9UHC1

Core Product Specifications and Parameters

Biological Background: MLH3 Function and Localization

• MLH3 is a member of the DNA mismatch repair MutL protein family, sharing homology with bacterial MutL and human MLH1.
• It is probably involved in the repair of DNA mismatches, contributing to genomic stability.
• MLH3 localizes to the nucleus, consistent with its role in DNA repair.
• The protein is ubiquitously expressed across tissues.
• Alternative splicing generates multiple MLH3 isoforms.
• Mutations in MLH3 are associated with hereditary nonpolyposis colorectal cancer (HNPCC), a form of Lynch syndrome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 1190–1389) of human MLH3; consider using the full-length recombinant protein as a positive control in ELISA.
• For Western blot, expect a band near 164 kDa; validate with known MLH3-expressing cell lines (e.g., human, mouse, rat).
• When using ELISA, optimize coating antigen concentration and antibody dilution to achieve a linear detection range.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, from concept to clinic. For DNA mismatch repair studies, we offer validated MLH3 antibodies, recombinant antigens, and ancillary reagents to streamline assay development. Explore our catalog for bulk sourcing and customized solutions tailored to your DNA repair assay needs.