MIXL1 Rabbit pAb for WB and ELISA - Q9H2W2

Core Product Specifications and Parameters

Biological Background: MIXL1 Function and Localization

• MIXL1 is a homeobox transcription factor critical for early embryonic development, driving axial mesendoderm morphogenesis and endoderm formation.
• It promotes differentiation of primitive streak cells into hematopoietic lineages, playing a key role in the expansion of hemangioblastic progenitors.
• The protein also contributes to heart and gut morphogenesis during embryogenesis, and acts as a negative regulator of brachyury expression.
• MIXL1 is localized exclusively in the nucleus, consistent with its function as a DNA-binding transcriptional regulator.
• Expression is highly restricted: in adults, it is found primarily in immature B- and T-lymphoid cells and secondary lymphoid tissues; in development, it is detected in differentiating embryonic stem cells.
• The gene undergoes alternative splicing, potentially yielding isoforms with distinct functional properties.
• Post-translational modifications include phosphorylation, as identified by proteomics, which may modulate its activity.
• Keywords associated with MIXL1 include: developmental protein, differentiation, DNA-binding, homeobox, nucleus, phosphoprotein, transcription regulator.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal region (aa 150–232) of human MIXL1; this design may provide specificity for endogenous MIXL1 in relevant samples.
• For Western blot, the predicted molecular weight is approximately 25 kDa; consider using appropriate gel percentages and transfer conditions for low-molecular-weight proteins.
• Cross-reactivity is reported for human, mouse, and rat; validate in the species and tissue of interest, especially given the restricted expression pattern.
• ELISA applications can be explored using the antibody as a capture or detection reagent; optimise coating and blocking conditions.
• Because MIXL1 is typically expressed at low levels, enrichment of nuclear fractions or use of a sensitive detection system may be beneficial.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimised monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your assay development. For researchers focused on developmental biology or stem cell markers like MIXL1, we facilitate access to hard-to-find raw materials and provide technical insights to streamline your workflow from concept to clinic. Let us bridge your supply needs with reliable, well-characterised immunological reagents.