Mitofusin 2 Rabbit mAb for WB - O95140

Core Product Specifications and Parameters

Biological Background: Mitofusin-2 Function and Localization

• Mitofusin-2 (MFN2) is a mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion, essential for maintaining mitochondrial network dynamics. Related references: PMID:11181170 PMID:11950885 PMID:19889647 PMID:28114303
• Overexpression of MFN2 induces the formation of mitochondrial networks, and membrane clustering requires GTPase activity and coiled coil domain rearrangements.
• MFN2 plays a central role in mitochondrial metabolism, regulation of apoptosis, and vascular smooth muscle cell proliferation.
• It is required for the clearance of damaged mitochondria through selective autophagy (mitophagy) and recruits PRKN to dysfunctional mitochondria. Related references: PMID:23620051
• MFN2 is involved in the unfolded protein response (UPR) upon ER stress, regulating EIF2AK3 activation and processes like autophagy and apoptosis.
• Subcellular localization: Mitochondrion outer membrane.
• Tissue specificity: Ubiquitously expressed at low levels, with high expression in heart and kidney.
• Keywords highlight roles in apoptosis, autophagy, and disease association with Charcot-Marie-Tooth disease type 2A (CMT2A).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-100) of human MFN2, a cytoplasmic domain; consider that the antibody may recognize an epitope within this region.
• For Western blot, a band near 86 kDa is expected; validate specificity using knockout cell lines or siRNA-mediated knockdown.
• In immunofluorescence (IF-P), appropriate permeabilization may be necessary to access the mitochondrial outer membrane; co-stain with mitochondrial markers to confirm localization.
• Cross-reactivity has been reported for human, mouse, and rat; if using other species, experimental verification is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a one-stop sourcing platform for IVD raw materials, including validated antibodies like this anti-MFN2 monoclonal antibody. Our extensive portfolio supports mitochondrial biology research, offering bulk supplies and customized procurement of rare-target reagents. With a commitment to quality and efficiency, CamelBio streamlines your path from concept to clinic.