MICAL1 Rabbit Polyclonal Antibody for WB, ELISA - Q8TDZ2

Core Product Specifications and Parameters

Biological Background: MICAL1 Function and Localization

• Encodes [F-actin]-monooxygenase MICAL1, also known as MICAL-1 or NEDD9-interacting protein with calponin homology and LIM domains.
• Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin to form methionine-sulfoxide, resulting in actin filament disassembly and preventing repolymerization. Related references: PMID:29343822
• In the absence of actin, functions as a NADPH oxidase producing H(2)O(2). Related references: PMID:21864500 PMID:26845023 PMID:29343822
• Acts as a negative regulator of apoptosis via interaction with STK38 and STK38L, antagonizing their activation by MST1/STK4.
• Involved in regulation of lamina-specific connectivity in the nervous system, such as the development of lamina-restricted hippocampal connections, through redox regulation of the actin cytoskeleton.
• May act as a Rab effector protein playing a role in vesicle trafficking; promotes endosomal tubule extension by associating with RAB8, RAB10, and GRAF proteins on the endosomal membrane. Related references: PMID:32344433
• Localizes to the cytoplasm, cytoskeleton, endosome membrane, and midbody.
• Expressed in thymus, lung, spleen, kidney, testis, and hematopoietic cells.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 718-1067 in the C-terminal region, which may help avoid cross-reactivity with other MICAL family members sharing N-terminal domains.
• For Western blot, consider the predicted molecular weight of ~118 kDa and validate detection in relevant tissue lysates such as thymus or testis.
• For ELISA, consider pairing with a detection antibody recognizing a distinct epitope to enable sandwich assay development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This MICAL1 product serves research in actin cytoskeleton dynamics, apoptosis, and neurobiology, while CamelBio’s sourcing capabilities extend to rare and emerging targets, helping bridge the gap from concept to clinical application.