MIB1/DIP1 Rabbit Monoclonal Antibody for WB, ELISA - Q86YT6

Core Product Specifications and Parameters

Biological Background: MIB1/DIP1 Function and Localization

• Encoded by the MIB1 gene, MIB1 (also known as DAPK-interacting protein 1/DIP-1, Mind bomb homolog 1, and ZZANK2) is a RING-type E3 ubiquitin ligase that plays a pivotal role in the Notch signaling pathway by mediating ubiquitination of Delta ligands, promoting their endocytosis.
• Exhibits dual regulatory functions in apoptosis: it ubiquitinates DAPK1 for proteasomal degradation, thereby antagonizing DAPK1-mediated anti-apoptotic effects and promoting TNF-induced apoptosis (by similarity).
• Functions at the centrosome by ubiquitinating centriolar satellite proteins CEP131, CEP290, and PCM1, leading to inhibition of primary cilium formation in proliferating cells.
• Mediates Lys-63-linked polyubiquitination of TBK1, which is thought to participate in TBK1 kinase activation and downstream signaling.
• During adenovirus infection, the ligase ubiquitinates the viral core-capsid bridging protein, facilitating genome delivery into the nucleus for infection.
• Localizes to multiple subcellular compartments, including the cytoplasm, cell membrane, and centrosome-associated centriolar satellites, reflecting its diverse functional roles.
• Widely expressed at low levels across tissues, with relatively higher expression in spinal cord, ovary, and all examined brain regions. The protein contains multiple zinc fingers, ANK repeats, and coiled-coil domains, and is classified as a phosphoprotein linked to cardiomyopathy disease variants.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-100) of human MIB1, a region that is likely conserved across species; this design may contribute to the antibody’s reported cross-reactivity with mouse and rat MIB1.
• For Western blotting, whole-cell lysates from human, mouse, or rat samples can be used; expect a band near 110 kDa. Given the protein’s typically low endogenous expression, consider enriching target signals via immunoprecipitation or optimizing detection conditions.
• In ELISA applications, the antibody can be explored as a capture or detection reagent; perform cross-validation experiments to confirm binding specificity in your particular assay format.
• When studying MIB1 functions in primary cilia or Notch signaling, ensure that sample preparation preserves relevant cellular localizations (e.g., centrosomes); inclusion of appropriate controls and reference antibodies is advisable.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in delivering integrated IVD raw material solutions to diagnostic developers, laboratories, and research institutes worldwide. This anti-MIB1/DIP1 monoclonal antibody supports investigations into ubiquitination pathways and Notch signaling, and complements our comprehensive portfolio of validated antibody pairs, recombinant proteins, and ancillary reagents. Whether you need bulk quantities, customized formulations, or assistance sourcing rare-target antibodies, CamelBio provides reliable, end-to-end procurement support tailored to your project needs.