MGMT Rabbit Polyclonal Antibody for WB and ELISA - P16455

Core Product Specifications and Parameters

Biological Background: MGMT Function and Localization

• MGMT (O6-methylguanine-DNA methyltransferase), also known as 6-O-methylguanine-DNA methyltransferase, is a suicide DNA repair enzyme that directly removes methyl groups from O6-methylguanine and O4-methylthymine in DNA.
• The repair mechanism involves stoichiometric transfer of the methyl group to a cysteine residue within the enzyme, leading to irreversible inactivation of MGMT.
• This protein plays a critical role in the cellular defense against the biological effects of alkylating agents, thereby contributing to genomic stability.
• Subcellular localization is primarily nuclear, consistent with its function in DNA repair.
• MGMT is a zinc-binding metalloprotein and undergoes phosphorylation events identified through proteomics.
• It is classified under methyltransferase, transferase, and DNA repair pathways, highlighting its role in direct DNA damage reversal.

Experimental Guidance and Technical Tips

• For Western blot, a specific band around 22 kDa corresponding to MGMT is expected; consider optimizing blocking conditions and antibody dilution for your particular samples.
• ELISA can be employed to quantify MGMT protein levels; it is advisable to validate the assay with known standard curves and appropriate controls.
• The antibody was raised against a recombinant fusion protein spanning amino acids 1-207 of human MGMT, which should provide good recognition of the target.
• Cross-reactivity is reported for human; if experiments involve other species, verify reactivity beforehand.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop sourcing solution for IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For DNA repair and cancer research targets like MGMT, we provide reliable access to high-quality raw materials and technical support from concept to clinic. Partner with us to explore customized sourcing and assay development needs.