mGluR2 Rabbit Monoclonal Antibody for WB - Q14416

Core Product Specifications and Parameters

Biological Background: mGluR2 Function and Localization

• mGluR2 (metabotropic glutamate receptor 2) is a dimeric G protein-coupled receptor encoded by the GRM2 gene in humans.
• Activated by the excitatory neurotransmitter L-glutamate, mGluR2 modulates synaptic transmission and neuronal excitability by inhibiting presynaptic calcium channels and reducing further glutamate release (PubMed:37286794). Related references: PMID:37286794
• Upon ligand binding, mGluR2 signals through G proteins to modulate down-stream effectors such as adenylate cyclase, potentially mediating suppression of neurotransmission, synaptogenesis, or synaptic stabilization.
• mGluR2 localizes primarily to the cell membrane, synapses, dendritic projections, and cell projections, consistent with its role in synaptic signaling.
• The receptor is widely expressed in different regions of the adult brain and fetal brain, with confirmed protein expression in the brain cortex.
• Key structural features include seven transmembrane helices, disulfide bonds, glycosylation, and a signal peptide, classifying it as a member of the G protein-coupled receptor family.
• Additionally, mGluR2 acts as a host entry factor for several viruses, including influenza, rabies, and SARS-CoV-2, by hijacking its endocytosis machinery.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal intracellular domain of human mGluR2; thus, the antibody is likely to recognize the full-length receptor and potentially its truncated isoforms in lysates.
• For Western blot (WB), a band around 96 kDa is expected; include appropriate negative and positive controls from relevant tissue or cell lines.
• Immunofluorescence (IF-P) may require permeabilization due to the intracellular epitope; consider antigen retrieval methods for paraffin-embedded sections.
• ELISA applications depend on the specific assay format; validate with recombinant mGluR2 protein as a standard.
• Cross-reactivity with mouse and rat enables comparative studies, but confirm specificity in each species' tissue.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with reliable access to high-quality IVD raw materials like this anti-mGluR2 monoclonal antibody, supporting neuroscience and synaptic biology studies. Our comprehensive portfolio includes validated antibody pairs, optimized recombinant proteins, and bulk ancillary reagents for assay development. With expert technical consultation, we help streamline your transition from concept to clinic.