METTL5 Rabbit Polyclonal Antibody for WB, ELISA - Q9NRN9

Core Product Specifications and Parameters

Biological Background: METTL5 Function and Localization

• METTL5 (Methyltransferase-like protein 5), also known as MRT72 or HSPC133, functions as the catalytic subunit of a heterodimer with TRMT112, specifically methylating the N6 position of adenine 1832 in 18S rRNA, a modification essential for translation and embryonic stem cell pluripotency and differentiation.
  Related references: PMID:31328227, PMID:32217665, PMID:33357433
• This methylation site resides in the decoding center of the ribosome, highlighting its critical role in translational accuracy.
• Subcellularly, METTL5 localizes predominantly to the nucleus and is also found at presynaptic and postsynaptic neuronal sites.
• METTL5 is expressed from very early brain development (8 post-conceptual weeks) and persists in adulthood in multiple brain substructures, including the cerebellar cortex, hippocampus, and striatum.
• Mutations in METTL5 are linked to intellectual disability, underscoring its importance in neurodevelopment.
• The enzyme belongs to the methyltransferase superfamily and utilizes S-adenosyl-L-methionine (SAM) as a methyl donor.

Experimental Guidance and Technical Tips

• The immunogen (aa 1-209) spans the conserved N-terminal region of METTL5, supporting the observed cross-reactivity to mouse and rat; validate in relevant sample species.
• For Western blot, a starting dilution of 1:500–1:2000 is suggested; optimize using nuclear-enriched lysates, as METTL5 is nuclear-localized.
• In ELISA applications, the polyclonal nature may offer broad epitope recognition; consider pairing with a monoclonal antibody for sandwich assays.
• The expected band size is ~24 kDa, but post-translational modifications or sample preparation may cause slight shifts; use appropriate molecular weight markers.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials. This METTL5 antibody supports research into rRNA modification, translation, and neurodevelopment. We offer a comprehensive portfolio—from optimized monoclonal and polyclonal antibodies to ancillary reagents and rare-target sourcing—to help streamline your assay development from concept to clinic.