METTL14 Rabbit pAb - Q9HCE5

Core Product Specifications and Parameters

Biological Background: METTL14 Function and Localization

• METTL14 forms a heterodimer with METTL3 to catalyze N6-methyladenosine (m6A) modification on mRNA, regulating circadian clock, embryonic stem cell differentiation, and cortical neurogenesis. Related references: PMID:24316715 PMID:24407421 PMID:25719671

• Within the METTL3-METTL14 complex, METTL14 acts as the RNA-binding scaffold recognizing substrate RNA, while METTL3 provides the catalytic core. Related references: PMID:27281194 PMID:27373337 PMID:27627798

• m6A modification occurs at the consensus motif 5'-[AG]GAC-3' and plays a critical role in mRNA stability and processing. Related references: PMID:24316715 PMID:24407421 PMID:25719671

• By similarity, m6A methylation regulates spermatogonial differentiation and meiosis, essential for male fertility, and promotes transcript destabilization in embryonic stem cells.

• METTL14 is a nuclear protein, annotated as a phosphoprotein and RNA-binding protein, and is part of the reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–210 of human METTL14, covering the N-terminal region potentially involved in complex formation; epitopes may be conserved across human, mouse, and rat.
• For Western blotting, expect a band near 52 kDa; optimize loading and dilution using positive controls such as HeLa or HEK293 lysates.
• In immunofluorescence and immunohistochemistry, validate nuclear localization; fixation and permeabilization conditions may influence staining.
• Polyclonal antibodies can show lot-to-lot variability; confirm specificity with knockdown controls or blocking peptides.
• For ELISA, optimize coating antigen and detection conditions; the recombinant immunogen may serve as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target procurement to support epitranscriptomic research and diagnostic development. This METTL14 antibody is part of our portfolio designed to accelerate m6A pathway studies and RNA modification assays. Leverage our one-stop supply chain to streamline your workflow from discovery to diagnostic application.