METRNL Rabbit Polyclonal Antibody for WB, ELISA - Q641Q3

Core Product Specifications and Parameters

Biological Background: Meteorin-like protein (METRNL) Function and Localization

• Meteorin-like protein (METRNL), also known as Subfatin, is a secreted hormone encoded by the METRNL gene. It undergoes alternative splicing, generating distinct isoforms with potential functional diversity.
• METRNL is induced by exercise in skeletal muscle or by cold exposure in adipose tissue and acts as a circulating factor that promotes energy expenditure.
• It stimulates the browning of white adipose tissue, enhancing thermogenic gene programs indirectly by recruiting immune cell subtypes into adipose tissue.
• METRNL triggers an eosinophil-dependent increase in IL-4 expression, leading to alternative activation of adipose tissue macrophages, which support thermogenic and anti-inflammatory gene expression.
• The protein is required for some cold-induced thermogenic responses, playing a role in metabolic adaptation to low temperatures.
• Subcellularly, METRNL is secreted into the extracellular space, consistent with its hormonal function.
• Tissue expression is highest in skeletal muscle, subcutaneous and epididymal white adipose tissue, heart, brown adipose tissue, and kidney.
• Keywords associated with METRNL include disulfide bond, signal peptide, and hormone, underscoring its secretory nature and structural features.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 179-311 of mouse METRNL. For optimal detection, consider using antigen retrieval methods appropriate for the target tissue and application.
• In Western blot, a band around 34 kDa corresponds to METRNL. Validate specificity in tissues with known high expression, such as skeletal muscle or adipose depots.
• For ELISA, this polyclonal antibody may serve as a capture or detection reagent; however, pairing with a monoclonal antibody may improve assay sensitivity and specificity. Validate in the relevant sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, research laboratories, and institutes with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For metabolism and energy expenditure research, we provide reliable access to antibodies targeting METRNL and other key metabolic regulators. From concept to clinic, CamelBio streamlines procurement and helps you source even rare-target raw materials efficiently.