Methyl-HP1 gamma/CBX3 (H175) Rabbit Polyclonal Antibody Q13185 for Dot Blot & ELISA

Core Product Specifications and Parameters

Biological Background: CBX3 (HP1 gamma) Function and Localization

CBX3, also known as HP1 gamma, is a component of heterochromatin that recognizes and binds histone H3 tails methylated at Lys-9, leading to epigenetic repression. It also binds histone H1.4 methylated at Lys-26 (H1.4K26me) and is excluded from chromatin when H1.4 is simultaneously methylated and phosphorylated. Related references: [PMID:11242053] [PMID:16127177]

Through interaction with MIS12 complex proteins, CBX3 is involved in the formation of functional kinetochores. It contributes to the conversion of local chromatin to a heterochromatin-like repressive state via H3 Lys-9 trimethylation and mediates the recruitment of methyltransferases SUV39H1/2 by the PER complex to circadian target genes such as PER2 and PER1. Related references: [PMID:15502821]

CBX3 also mediates the recruitment of NIPBL to sites of DNA damage at double-strand breaks, highlighting a role in DNA repair. Related references: [PMID:28167679]

Subcellularly, CBX3 is a nuclear protein that associates with euchromatin and is largely excluded from constitutive heterochromatin. It may also be associated with microtubules and mitotic poles during mitosis. Related references: [PMID:28977666]

Post‑translational modifications of CBX3 include N‑terminal acetylation, multiple lysine acetylations, and phosphorylation at several serine residues (Ser93, Ser95, Ser97, Ser99, Ser176), which regulate its function and interactions. Related references: [PMID:19608861] [PMID:16964243] [PMID:20068231] [PMID:21406692] [PMID:18669648] [PMID:24275569] [PMID:23186163]

Experimental Guidance and Technical Tips

• The immunogen covers the N‑terminal region (aa 1‑183) of human CBX3; consider verifying epitope recognition when using truncation mutants or isoform-specific assays.
• For dot blot (DB), optimize blocking and antibody dilution to minimize non‑specific binding to the membrane. A pilot titration is recommended.
• For ELISA, ensure the antigen coating matches the methylated epitope of interest. Validate the assay in the relevant sample system (e.g., nuclear extracts from human cell lines).
• Nuclear extraction protocols should be employed to enrich CBX3, as it is predominantly a nuclear protein. Avoid harsh denaturing conditions that may alter methyl‑specific epitope recognition.
• Pair with appropriate controls (e.g., recombinant methylated vs. unmethylated CBX3 peptides) to confirm specificity for the methyl‑H175 modification.
• This antibody has been tested only on human samples; cross‑reactivity with other species has not been evaluated. Validate in the target species before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one‑stop platform for IVD raw‑material sourcing, providing diagnostic manufacturers and research institutes with access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare‑target raw materials. For epigenetic/chromatin research focusing on HP1 gamma/CBX3, we can support your project with the methyl‑specific or pan‑specific CBX3 antibodies described, as well as matching antigen standards and technical consultation. Let CamelBio streamline your transition from concept to clinic with reliable, high‑quality reagents and dedicated supply chain solutions.