METAP2 Rabbit Monoclonal Antibody for WB, ELISA - P50579

Core Product Specifications and Parameters

Biological Background: METAP2 Function and Localization

• METAP2 encodes methionine aminopeptidase 2, also known as initiation factor 2-associated 67 kDa glycoprotein (p67, p67eIF2) and peptidase M, which plays a dual role in protein maturation and translational control.
• It cotranslationally removes the N-terminal methionine from nascent proteins when the second residue is small and uncharged (e.g., Ala, Cys, Gly, Pro, Ser, Thr, Val). Its catalytic activity toward Met-Val peptides is over 100-fold higher than METAP1, indicating a primary responsibility for processing Met-Val and Met-Thr termini in vivo.
• Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by kinases such as EIF2AK2/PKR and EIF2AK1/HCR, thereby critically regulating protein synthesis under stress conditions.
• Primarily localizes in the cytoplasm, consistent with its role in cotranslational processing and eIF2 protection.
• Belongs to the peptidase M24A family; features metal-binding capacity and is post-translationally modified by acetylation, phosphorylation, and glycosylation.
• Alternative splicing generates multiple isoforms, potentially contributing to tissue-specific or context-dependent regulation of protein synthesis.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 380–478 in the C-terminal region of METAP2; this region is conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blotting, METAP2 has a predicted molecular weight of ~53 kDa, but glycosylation or other modifications may result in slight band shifts; validate using appropriate positive controls (e.g., HeLa, HEK293, or tissue lysates) and consider using a reducing gel.
• In ELISA applications, optimization of coating antigen concentration and antibody dilution is recommended; the antibody may capture both full-length and certain truncated/natural isoforms of METAP2.
• Because METAP2 functions in both methionine excision and eIF2 protection, this antibody is suitable for studies on translation initiation, protein maturation, and cellular stress responses.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a single point of access to high-quality IVD raw materials and technical support for methionine aminopeptidase 2 and other translational control targets. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized rare-target sourcing solutions to streamline your assay development from concept to clinic. Whatever your project requires, CamelBio is ready to help you bridge the gap between discovery and diagnostic application.