Merlin Rabbit Polyclonal Antibody for WB, ELISA - P35240

Core Product Specifications and Parameters

Biological Background: Merlin Function and Localization

• Merlin is a member of the ERM (ezrin-radixin-moesin) protein family and acts as a tumor suppressor, encoded by the NF2 gene.
• It functions as a probable regulator of the Hippo/SWH signaling pathway, which restricts proliferation and promotes apoptosis, thereby suppressing tumorigenesis.
• Merlin may act as a membrane stabilizing protein and inhibits PI3 kinase signaling by binding to AGAP2.
• It suppresses cell proliferation by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex.
• Subcellular localization includes the cell membrane (filopodia and ruffles), cytoplasm (perinuclear region, cytoskeleton, and granules), and nucleus.
• Widely expressed in tissues; multiple isoforms exist with predominant expression of isoforms 1 and 3.
• Keywords associated with Merlin include alternative splicing, deafness, disease variant, phosphoprotein, tumor suppressor, and Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (aa 347-595) of human Merlin; consider that this region may be conserved across species for cross-reactivity.
• For Western blot (WB), a band around 70kDa is expected; validate in the relevant sample system with appropriate controls.
• Due to the polyclonal nature, multiple bands or cross-reactivity with other ERM family members may occur; verify specificity via knockdown or specific inhibitors.
• For ELISA, optimize coating conditions and antibody dilutions based on the specific assay format.
• This antibody is suitable for detecting Merlin in human, mouse, and rat samples; however, perform individual validation for each species and application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive supply chain for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. For targets like Merlin in tumor suppression and Hippo signaling, we provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Partner with us to streamline your diagnostic development with reliable raw-material supply.