Menin Rabbit Monoclonal Antibody for WB, ELISA - O00255

Core Product Specifications and Parameters

Biological Background: Menin Function and Localization

• Menin, also known as MEAI or SCG2, is a tumor suppressor protein encoded by the MEN1 gene, with mutations associated with multiple endocrine neoplasia type 1 (keyword: Disease variant).
• It is an essential component of the MLL/SET1 histone methyltransferase complex, which specifically methylates lysine 4 of histone H3 (H3K4), thereby regulating chromatin structure and transcription.
• Menin acts as a transcriptional regulator: it binds the TERT promoter to repress telomerase expression, modulates TGFB1/SMAD3 signaling to inhibit cell proliferation, and represses JUND- and RELA-mediated transcriptional activation.
• Positively regulates HOXC8 and HOXC6 gene expression, and may contribute to normal hematopoiesis through activation of HOXA9.
• Localizes to the nucleus and is ubiquitously expressed across tissues.
• Key post-translational modifications include phosphorylation (keyword: Phosphoprotein), and the protein undergoes alternative splicing.
• The canonical isoform has a calculated molecular weight of approximately 67.5 kDa (UniProt), consistent with the observed 68 kDa band in Western blot.

Experimental Guidance and Technical Tips

• For Western blot, the antibody detects a band at ~68 kDa. Verify specificity by using positive and negative control cell lysates (e.g., MEN1 wild-type vs. knockout).
• The immunogen peptide spans amino acids 516–615 (C-terminal region). Confirm that the epitope is conserved in your species of interest and check for potential splice variants that may alter migration or detection.
• In ELISA, use recombinant Menin protein or peptide as standard. Optimize antibody dilution and coating conditions for best signal-to-noise ratio.
• Reported cross-reactivity with mouse and rat Menin suggests suitability for rodent models; however, validate in your specific application.
• For immunoprecipitation or other untested applications, protocol optimization may be required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research labs from concept to clinic. For your Menin antibody and epigenetic research needs, we offer validated antibodies, bulk ancillary reagents, and customized sourcing for rare targets. Our integrated supply chain ensures reliable access to high-quality reagents, enabling seamless assay development and production.