MEK3/MEK6 Rabbit Monoclonal Antibody for WB and ELISA - P46734 / P52564

Core Product Specifications and Parameters

Biological Background: MEK3/MEK6 Function and Localization

• MEK3 (MAP2K3) is a dual specificity mitogen-activated protein kinase kinase that activates p38 MAP kinase by phosphorylating threonine and tyrosine residues, serving as a critical mediator in cellular responses to stress and inflammatory cytokines.
• It plays a central role in signal transduction cascades initiated by environmental stressors and cytokines, leading to downstream effects such as regulation of gene expression, cell differentiation, and apoptosis.
• MEK3 is part of a specific signaling pathway where activation of the adrenergic receptor ADRA1B triggers the MAP kinase cascade, ultimately activating MAPK14 (p38α).
• The protein is widely expressed in human tissues, with particularly high abundance in skeletal muscle, reflecting its importance in muscle physiology and stress adaptation.
• Post-translational modifications, including phosphorylation and acetylation, tightly regulate MEK3 kinase activity and its interaction with upstream and downstream partners.
• MEK6 (MAP2K6) is a closely related kinase that shares functional redundancy with MEK3 in phosphorylating and activating p38 MAP kinases, and their combined roles are essential for full stress-responsive signaling.
• Genetic variations in the MAP2K3 gene have been associated with disease phenotypes, underscoring its potential clinical significance.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weights of MEK3 and MEK6 are approximately 39 kDa and 37 kDa, respectively; validate detection in your sample type using appropriate positive and negative controls.
• The immunogen peptide corresponds to amino acids 248–347 of human MEK3 (P46734), which shares homology with MEK6, supporting cross-reactivity; confirm specificity in your experimental system.
• When using this antibody in ELISA, carefully optimize the coating and detection conditions to achieve optimal signal-to-noise ratios.
• Given reported cross-reactivity with mouse and rat, this antibody is suitable for translational studies; however, always verify performance in the species of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with one-stop access to IVD raw materials, including validated antibody pairs and monoclonal antibodies like this MEK3/MEK6 rabbit mAb, ideal for studying MAP kinase signaling. Our comprehensive portfolio covers critical targets in stress and inflammation pathways, ensuring reliable sourcing for assay development. We also provide bulk ancillary reagents and customized solutions for rare target raw-material sourcing, helping you accelerate from concept to clinic.