MEK3 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P46734

Core Product Specifications and Parameters

Biological Background: MEK3 Function and Localization

• MEK3 (MAP kinase kinase 3), also known as MKK3, MAPKK3, PRKMK3, or SAPKK2, is a dual specificity protein kinase that belongs to the MAP kinase kinase family.
• It is activated by various cytokines and environmental stressors in vivo, serving as a critical mediator of the cellular stress response.
• Upon activation, MEK3 catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38 (MAPK14), thereby propagating downstream signaling.
• MEK3 is part of a signaling cascade initiated by the activation of the adrenergic receptor ADRA1B, ultimately leading to MAPK14 activation and modulation of gene expression, cell survival, and inflammation.
• The protein is highly expressed in skeletal muscle but is widely distributed across many other tissues, reflecting its broad regulatory functions.
• Alternative splicing generates multiple transcript variants, and the kinase domain contains motifs for ATP binding and phosphotransferase activity.
• Post-translational modifications include acetylation and phosphorylation, which may regulate MEK3 activity and interactions within the MAPK pathway.

Experimental Guidance and Technical Tips

• This antibody was raised against an N-terminal fragment (amino acids 1–70) of human MEK3, making it potentially useful for detecting full-length MEK3 and isoforms sharing this region; consider validating specificity in your model system.
• For Western blotting, a band at approximately 39 kDa is expected; optimize loading controls and consider the impact of post-translational modifications on migration.
• In IHC-P, antigen retrieval and permeabilization steps may require optimization for reliable detection of MEK3 in formalin-fixed paraffin-embedded tissues.
• For IF/ICC, appropriate fixation and blocking conditions are critical; co-staining with organelle markers can help confirm subcellular localization.
• ELISA detection can be performed following standard protocols; including positive and negative controls is recommended to assess assay performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers streamlined access to a comprehensive portfolio of IVD raw materials, including validated antibodies such as this MEK3 rabbit pAb. From optimized monoclonal and polyclonal antibodies to bulk ancillary reagents, we support your MAP kinase signaling research and assay development with reliable, one-stop sourcing solutions. Our expertise spans the entire diagnostic pipeline, helping you accelerate from concept to clinic with confidence.