MEGF10 Rabbit pAb - Q96KG7

Core Product Specifications and Parameters

Biological Background: MEGF10 Function and Localization

• MEGF10 is a membrane receptor that mediates phagocytosis of apoptotic cells by macrophages and astrocytes, recognizing phosphatidylserine via C1q. Related references: PMID:27170117

• Cooperates with ABCA1 in engulfment and promotes large intracellular vacuole formation, potentially involved in amyloid-beta peptide uptake. Related references: PMID:17643423, PMID:20828568

• Essential for astrocyte-dependent clearance of apoptotic neurons in the developing cerebellum. Related references: PMID:27170117

• Plays a role in muscle cell proliferation, adhesion, and motility, and is crucial for myogenesis regulation via the Notch signaling pathway. Related references: PMID:28498977

• May function in retinal neuron mosaic spacing through homotypic repulsion to ensure even distribution across the retina. Related references: PMID:17498693, PMID:17643423, PMID:20828568

• Subcellularly localizes to the cell membrane and phagocytic cup.

• Contains multiple EGF-like domains, is glycosylated, phosphorylated, and subject to ubiquitin conjugation.

• Expressed in muscle tissue at the protein level; linked to muscle disorders like EMARDD.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 26-160) of human MEGF10. This antibody may detect isoforms containing this sequence; consider alternative splicing in your target system.
• For Western blot, the predicted molecular weight is ~122 kDa. Use appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• Cross-reactivity with human, mouse, and rat has been reported. Validate specificity and sensitivity in your specific sample matrix before routine use.
• As a polyclonal antibody, lot-to-lot consistency should be verified for quantitative applications like ELISA. Consider initial titration experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides IVD manufacturers and research laboratories with streamlined access to high-quality raw materials, including validated polyclonal antibodies, antigen-grade recombinant proteins, and ancillary reagents. Our portfolio covers niche targets like MEGF10, supporting applications from muscle disease research to phagocytosis assays. Leverage our one-stop sourcing to accelerate your assay development from concept to clinic.