MED25 Rabbit pAb - Q71SY5

Core Product Specifications and Parameters

Biological Background: MED25 Function and Localization

• MED25 is a subunit of the Mediator complex, a multisubunit coactivator essential for regulated transcription of nearly all RNA polymerase II-dependent genes.
• It acts as a molecular bridge, relaying signals from gene-specific transcription factors to the basal transcription machinery, thereby facilitating the assembly of a functional preinitiation complex.
• MED25 is specifically required for RARA/RXRA-mediated transcription, linking it to retinoic acid signaling pathways.
• The protein is also known as Activator-recruited cofactor 92 kDa component (ARC92), Activator interaction domain-containing protein 1 (ACID1), and p78, reflecting its functional association with transcriptional activation.
• Encoded by the MED25 gene, alternative splicing yields multiple isoforms, which may contribute to functional diversity in different cell types.
• Subcellular localization is strictly nuclear, consistent with its role in transcription regulation and chromatin interaction.
• MED25 is ubiquitously expressed, with the highest mRNA levels found in brain, heart, kidney, peripheral leukocytes, placenta, skeletal muscle, and spleen.
• Mutations in MED25 are implicated in neurological disorders such as Charcot-Marie-Tooth disease type 2B2 (CMT2B2) and intellectual disability, highlighting its importance in the nervous system.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against a recombinant protein spanning amino acids 30–200 of human MED25. It is expected to detect endogenous MED25 at ~78 kDa in Western Blot and specific nuclear signals in IF/ICC.
• For WB, consider using fresh lysates and standard SDS-PAGE conditions; a reducing agent and boiling may be applied based on your sample requirements.
• For IF/ICC, perform fixation and permeabilization appropriate for nuclear targets, and validate localization in your cell line or tissue of interest.
• In ELISA applications, the antibody may be used as a capture or detection reagent following proper optimization of coating and blocking conditions.
• Cross-reactivity with mouse and rat MED25 is indicated; however, sequence alignment suggests potential recognition in other species, though verification is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement partner for in-vitro diagnostic manufacturers and research laboratories, offering streamlined access to validated raw materials including this MED25 polyclonal antibody. With expertise in transcriptional regulation research, we also supply ancillary reagents, recombinant proteins, and can assist in sourcing custom monoclonal or polyclonal antibodies to meet specific project needs. Whether you require bulk quantities or specialized raw-material consulting, CamelBio provides a reliable supply chain from discovery to clinical application.