MDH2 Rabbit pAb - P40926

Core Product Specifications and Parameters

Biological Background: MDH2 Function and Localization

• MDH2 encodes mitochondrial malate dehydrogenase, a key enzyme in the tricarboxylic acid (TCA) cycle that catalyzes the NAD+-dependent interconversion of malate to oxaloacetate (PubMed:27989324). Related references: PMID:27989324
• This reaction is central to cellular respiration and energy production, driving the mitochondrial electron transport chain (PubMed:35366151). Related references: PMID:35366151
• MDH2 also binds RNA, including cytosolic tRNAs and mRNAs, indicating non-canonical functions in RNA biology (PubMed:38609156). Related references: PMID:38609156
• It participates in the malate-aspartate shuttle, transferring reducing equivalents between the cytosol and mitochondria to maintain NAD+/NADH redox balance (By similarity).
• The protein localizes to the mitochondrial matrix, consistent with its metabolic roles.
• Post-translational modifications include acetylation, phosphorylation, glycosylation, and palmitoylation, which may regulate its activity or localization.
• Clinically, mutations in MDH2 are associated with developmental and epileptic encephalopathy (DEE51), classified as a disease variant and epilepsy-related gene.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band at ~36 kDa corresponding to the mature MDH2 protein. Enrichment of mitochondrial fractions is recommended to enhance signal specificity.
• Given the high sequence conservation between human and mouse MDH2, the antibody cross-reacts with both species. Suitable sample sources include HeLa, HEK293, and mouse liver or heart lysates.
• In ELISA applications, indirect ELISA using the recombinant immunogen (aa 59-338) as coating antigen can be used; optimize coating buffer and antibody dilution for best performance.
• Include appropriate mitochondrial and cytosolic controls to confirm specificity, as MDH2 is a mitochondrial matrix protein.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material platform, CamelBio streamlines procurement for diagnostic manufacturers and researchers, offering this anti-MDH2 polyclonal antibody as part of a comprehensive portfolio for metabolic and mitochondrial research. Our supply network covers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets—empowering your workflow from concept to clinic.